Mycobacteria Tuberculosis PCR with Rifampin Resistance (includes culture and stain)
Label Mnemonic: | TBPCR |
Epic code: | LAB7618 |
Order form: | Microbiology Laboratory Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
CPT code: | 87556 |
Specimen(s):
Sputum, Induced Sputum, Bronchoalveolar Lavage or Bronchial Washings
Collection Medium:
Sterile container |
Minimum:
5-15 mL in a sterile container. Pooled specimens are
unacceptable.
Rejection Criteria:
More than 1 specimen/day or 3 specimen/week.
Non-respiratory specimens. Repeat testing subsequent to a positive test.
Non-respiratory specimens. Repeat testing subsequent to a positive test.
Testing
Schedule:
0700-1630, 7 days a week, including holidays.
Turn Around
Time:
24 hours (upon receipt in laboratory)
Comments:
The Xpert MTB/RIF Assay is intended for use with specimens from
patients for whom there is clinical suspicion of tuberculosis and who
have received no therapy or less than 3 days of antituberculosis
therapy. This test does not provide confirmation of rifampin
susceptibility since mechanisms of rifampin resistance other than
those
detected by this device may exist. The Xpert MTB/RIF Assay must be
used in conjunction with a mycobacterial culture to address the risk
of
false negative results and to recover organisms for further testing
and
drug susceptibility testing.
This test is for diagnostic purposes and is not a test of cure. Repeat testing after initial positive test will require approval of the pathology resident on call or pager 4290.
This test is for diagnostic purposes and is not a test of cure. Repeat testing after initial positive test will require approval of the pathology resident on call or pager 4290.
Methodology:
PCR amplification: Xpert MTB/RIF Assay (Cepheid)
Sample
Processing:
Label transport tube with two patient identifiers, date and time of collection.
Specimen should be collected and packaged as close to shipping time as possible.
If transport is delayed, store specimen at 2-8°C (stable for up to 7 days). Alternatively, specimens can be kept at room temperature (35°C) for up to 72 hours.
Specimen should be collected and packaged as close to shipping time as possible.
If transport is delayed, store specimen at 2-8°C (stable for up to 7 days). Alternatively, specimens can be kept at room temperature (35°C) for up to 72 hours.
Transport
Instructions:
Use refrigerated coolant packs.
Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
CPT Code:
87556