HSV Qualitative PCR
Label Mnemonic: | HSVPCR |
Epic code: | LAB2467 |
Order form: | Microbiology/Molecular Infectious Disease Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
CPT code: | 87529 |
Specimen(s):
Vesicle Fluid, Surface Swab (in UTM; Throat, Buccal Mucosa, Cervical or Genital Lesions), Eye swab
samples collected in UTM or CSF
Collection Medium:
Minimum:
Collect one of the following specimens:
Vesicle fluid/swab (first three days of rash) in viral transport media
-OR-
0.5 mL CSF in CSF tube from the collection kit
Vesicle fluid/swab (first three days of rash) in viral transport media
-OR-
0.5 mL CSF in CSF tube from the collection kit
Rejection Criteria:
Sputum, tracheal aspirate or skin scrapings.
Vitreous fluid samples - Order mailout test Herpes Simplex Virus PCR, Vitreous (LAB7468).
Vitreous fluid samples - Order mailout test Herpes Simplex Virus PCR, Vitreous (LAB7468).
Testing
Schedule:
0800-1630 Monday through Friday.
Turn Around
Time:
24 hours (upon receipt in laboratory)
Reference Range:
Negative
Positive results will be reported as positive for HSV Type 1, HSV Type 2, or both.
Positive results will be reported as positive for HSV Type 1, HSV Type 2, or both.
Comments:
The performance characteristics of this test were determined by the
University of Iowa Microbiology and Molecular Pathology Laboratory.
It has not been cleared or approved by the U.S. Food and Drug
Administration (FDA). The FDA has determined that such clearance or
approval is not necessary. This test is for clinical purposes. It
should not be regarded as investigational or for research. The
laboratory is certified under the Clinical Laboratory Improvement
Amendments of 1988 (CLIA) as qualified to perform high complexity
clinical laboratory testing.
Methodology:
PCR amplification
Instructions:
Immediately place vesicle fluid, swab in viral transport
media. Source of specimen is required.
Sample
Processing:
Immediately place vesicle fluid or swab specimen into vial of transport media and seal tightly.
Label transport tube with two patient identifiers, date and time of collection.
Indicate specimen/fluid type on requisition.
Label transport tube with two patient identifiers, date and time of collection.
Indicate specimen/fluid type on requisition.
Sample
Storage:
Refrigerate.
Specimen cannot be frozen.
Specimen cannot be frozen.
Transport
Instructions:
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Transport in cooler with refrigerated coolant packs.
CPT Code:
87529
See also:
Herpes Simplex Virus PCR, Amniotic Fluid
Herpes Simplex Virus PCR, Amniotic Fluid