Epstein-Barr Virus Acute Panel
| Label Mnemonic: | EBVACUTE |
| Epic code: | LAB7810 |
| Order form: | Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 86308 (Heterophile), 86665 (VCA IgM) |
Specimen(s):
Plasma
Collection Medium:
 |
| Plasma Separator Tube 4.5 mL |
Minimum:
3.0 mL whole blood or TWO Microtainer® devices.
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around
Time:
3 hours (upon receipt in laboratory)
Reference Range:
Reference range for both analytes (Heterophile IgM, VCA IgM): 0.8 AI or less: Negative 0.9-1.0 AI: Indeterminate 1.1 AI or greater: Positive
Interpretive Data:
This panel consists of two tests for acute EBV infection: Heterophile IgM antibodies Viral capsid antigen (IgM) antibody Epstein-Barr virus (EBV) is the etiologic agent of infectious mononucleosis. EBV infection can be difficult to diagnose by laboratory testing; however, the majority of acute infections can be recognized by testing the patient's serum for heterophile antibodies (e.g., by the "Monospot" latex agglutination assay), which usually appear within the first 3 weeks of illness, but then decline rapidly within a few weeks. Heterophile antibodies are found in a very high percentage of infants and older children with acute mononucleosis. However, heterophile antibodies do not develop in approximately 10% of adults. Presence of VCA IgM antibodies indicates recent primary infection with EBV. Infection with EBV usually occurs early in life, typically as infectious mononucleosis. Other disorders due to EBV infection have been recognized, including Burkitt's lymphoma and nasopharyngeal carcinoma. EBV infection may also cause lymphoproliferative syndromes, especially in patients who have undergone renal or bone marrow transplantation and in those who have AIDS. Acute EBV infection usually shows either: Heterophile IgM positive / VCA IgM positive or Heterophile IgM negative / VCA IgM positive Heterophile IgM is relatively less sensitive than VCA IgM in acute EBV infection. The pattern of heterophile IgM positive / VCA IgM negative is very uncommon and difficult to interpret.
Comments:
Reference:
Klutts JS et al. Evidence-based approach for interpretation of Epstein-Barr virus serological patterns. J. Clin. Microbiol. 47(10): 3204-3210.
Klutts JS et al. Evidence-based approach for interpretation of Epstein-Barr virus serological patterns. J. Clin. Microbiol. 47(10): 3204-3210.
Methodology:
Multiplex Flow Immunoassay
Sample
Processing:
Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Sample
Storage:
Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
Transport
Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
86308 (Heterophile), 86665 (VCA IgM)