University of Iowa
Diagnostic Laboratories
(UIDL) Test Directory

319-384-7212 (local)
1-866-844-2522 (toll free)


Bilirubin, Direct Order Code: BILD
Order Form: Laboratory Requisition
Specimen:
Plasma
Collection Medium:
Plasma separator tube
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection 
containers.
Minimum:
2 mL whole blood from light green top tube or 1 light green microtube 
for pediatric patients
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
1 hour (upon receipt in laboratory)
Reference Range:
0.0 - 0.2 mg/dl
Comments:
Avoid hemolysis and unnecessary exposure to light.
Test
Limitations:
Hemolysis: Hemolysis interferes with the assay.

Lipemia: Roche/Hitachi 917/MODULAR analyzers - Lipemia levels greater 
than 100 mg/dl (Intralipid) cause significant (greater than 10%) 
positive interference to elevated
direct bilirubins.
Methodology:
Spectrophotometric
Sample
Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Sample
Storage:
Refrigerate.
Transport
Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
82248
 
See Additional Information:
Chemistry Pediatric Reference Ranges

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Updated: 10/11/2007