Bilirubin, Direct
Order Code: BILD
Order Form: Laboratory Requisition
Specimen:
Plasma
Collection Medium:
Plasma Separator Tube
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood from light green top tube or 1 light green microtainer for pediatric patients
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
1 hour (upon receipt in laboratory)
Reference Range:
0.0 - 0.2 mg/dl
Comments:
Avoid hemolysis and unnecessary exposure to light.
Test Limitations:
Hemolysis: Hemolysis interferes with the assay. Lipemia: Roche/Hitachi 917/MODULAR analyzers - Lipemia levels greater than 100 mg/dl (Intralipid) cause significant (greater than 10%) positive interference to elevated direct bilirubins.
Methodology:
Spectrophotometric
Sample Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Sample Storage:
Refrigerate.
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
82248
 
See also:
Bilirubin, Direct-Other, Body Fluid (e.g., surgical drain, pericardial, peritoneal, peritoneal dialysis, pleural, or synovial)