Bilirubin, Direct

Label Mnemonic:	BILD
Epic code:	LAB52
Order form:	Laboratory Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	82248

Specimen(s):

Plasma

Collection Medium:

Plasma Separator Tube 4.5 mL

Alternate Collection Media:

Call laboratory for additional acceptable specimen collection containers.

Minimum:

3 mL whole blood from light green top tube or ONE light green Microtainer^® for pediatric patients

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Turn Around Time:

1 hour (upon receipt in laboratory)

Reference Range:

0.0 - 0.2 mg/dL

Comments:

Avoid hemolysis and unnecessary exposure to light.

Test Limitations:

Hemolysis: No significant interference up to an H index of 1000 
(approximate hemoglobin concentration: 1000 mg/dL).

Lipemia levels greater than 1000 mg/dL (Intralipid) cause significant 
(greater than 10%) positive interference to elevated direct 
bilirubins.

Methodology:

Spectrophotometric

Instructions:

Avoid hemolysis and unnecessary exposure to light.

Sample Processing:

Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.

Sample Storage:

Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).

Transport Instructions:

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

82248

See also:
Bilirubin, Direct-Other, Body Fluid