Specimen:

Plasma

Collection Medium:

Alternate
Collection Media:

Call laboratory for additional acceptable specimen collection 
containers.

Minimum:

2 mL whole blood from light green top tube or 1 light green microtube 
for pediatric patients

Testing
Schedule:

24 hrs/day, 7 days a week, including holidays.

Analytic Time:

1 hour (upon receipt in laboratory)

Reference Range:

0.0 - 0.2 mg/dl

Comments:

Avoid hemolysis and unnecessary exposure to light.

Test
Limitations:

Hemolysis: Hemolysis interferes with the assay.

Lipemia: Roche/Hitachi 917/MODULAR analyzers - Lipemia levels greater 
than 100 mg/dl (Intralipid) cause significant (greater than 10%) 
positive interference to elevated
direct bilirubins.

Methodology:

Spectrophotometric

Sample
Processing:

Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.

Sample
Storage:

Refrigerate.

Transport
Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

82248

See Additional Information:
Chemistry Pediatric Reference Ranges