E. Coli Shiga toxin by EIA
| Order Code: | C EHEC |
| Order Form: | A-1a Clinical Microbiology Laboratory Requisition |
Specimen:
Stool
Collection Medium:
| Sterile container |
Minimum:
Submit 10-20 g stool in sterile container.
Testing
Schedule:
0700-2200, 7 days a week, including holidays.
Reference Range:
No Shiga-toxin-detected.
Comments:
This assay is a rapid in vitro microwell EIA for the detection of Shiga
toxins I and II (verotoxins) in stool specimens. The test is intended
for use as an aid in the diagnosis of enterohemorrhagic Escherichia
coli (EHEC) infection. Shiga toxin testing of stool specimens provides
better sensitivity than standard culture methods for the E. coli
O157:H7 serotype and non-O157 serotypes. Testing of broth cultures,
rather than the stool specimens themselves, is performed because the
amount of free fecal Shiga toxin in stools is often low. All Shiga
toxin positive broths (or presumptive E. coli O157:H7 isolates) will be
forwarded to the University Hygienic Laboratory for confirmatory
testing and genetic characterization. Shiga toxin testing is included
in routine stool cultures as recommended by Centers for
Disease Control (CDC).
Methodology:
Enzyme Immunoassay
Instructions:
Label specimen with two patient identifiers, date and time of
collection.
Sample
Storage:
Refrigerate.
Transport
Instructions:
Transport in cooler with refrigerated coolant packs.
CPT Code:
87427
See Additional Information:
Microbiology Specimen Collection and Transport
Microbiology Specimen Collection and Transport