EGFR Gene Analysis with Interpretation

Label Mnemonic:	EGFR
Epic code:	LAB4821
Order form:	Molecular Oncology Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	81235

Specimen(s):

Formalin fixed, paraffin embedded tissue block and 10 unstained 5-10 μM slides at 20-25°C and an H&E slide of the tissue

Minimum:

Tumor cells more than 50% of the total tissue and greater than 10mm² in surface area on the block

Rejection Criteria:

Specimens fixed in B5 fixative or that have been decalcified will not be accepted. Tumor specimens containing less than 50% tumor cells or are less than 10mm² in area may be unacceptable.

Testing Schedule:

Weekly

Turn Around Time:

7-10 working days

Reference Range:

Negative

Methodology:

PCR followed by DNA Sequencing

Instructions:

Submit formalin fixed, paraffin embedded tissue block or 10 unstained
5-10 μM slides and an H&E slide along with the requisition. Please include surgical pathology report and relevant clinical information.

Sample Processing:

Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Formalin-fixed, paraffin embedded tissue should be submitted.

Sample Storage:

Tissue Specimen - Freeze immediately and store frozen or send Formalin-fixed paraffin embedded blocks.

Transport Instructions:

Frozen Tissue - Ship frozen on dry ice. Ship express mail with overnight delivery. Formalin-fixed paraffin embedded blocks ship at room temperature.
Place requisition into outside pocket of bag.
Protect block or slides from excessive heat and ship in cooled container during summer months.

CPT Code:

81235