Cytomegalovirus (CMV) Quantitation by PCR
Order Code: CMVPCR
Order Form: Microbiology/Molecular Pathology Infectious Disease Requisition
Specimen:
Whole Blood
Collection Medium:
Pink top tube
Minimum:
Adult Preferred Minimum: 5 mL whole blood from pink top (K2-EDTA) tube Adult Absolute Minimum: 3 mL whole blood from pink top (K2-EDTA) tube Pediatric Minimum: 1 mL whole blood from pink top (K2-EDTA) tube
Testing Schedule:
Batch analysis performed daily Monday through Friday. Sample must be received by the Molecular Pathology Laboratory by 1300 for same day testing. Specimens received on the weekend are analyzed on Monday.
Analytic Time:
1-4 days
Reference Range:
Negative
Reportable linear range of 200 - 2,000,000 copy/mL
Comments:
Specimens stored at 4°C will be accepted up to 72 hours after collection.

Testing requires a dedicated tube.
Methodology:
Real-Time Polymerase Chain Reaction (PCR)
Instructions:
Sample Processing: Centrifuge for 20 minutes at 1600 x g within 4 hours of collection. Aseptically enter the tube and aliquot plasma into sterile RNase and DNase free tubes. Store at 2-8°C for 72 hours or -20 to -80°C long term. For CSF specimens aliquot into RNase and DNase free tubes and store at 2-8°C for 72 hours or -20 to -80°C for long term. Transport Instructions: Place coolant packs into styrofoam container. Place requisition into outside pocket of bag. Protect specimen by wrapping in bubble-wrap or toweling. Recommend express mail or equivalent if not on courier service.
CPT Code:
87497