JAK2 V617F Mutation Detection Assay, Whole Blood

JAK2 V617F Mutation Detection Assay

Order Code:	JAK2
Order Form:	Molecular Oncology Requisition

Specimen:

Whole Blood

Collection Medium:

Lavender top tube 3 mL (EDTA)

Minimum:

Adults 3 mL whole blood. Testing on smaller volumes than those requested will be attempted. However, in some cases, small blood volumes may compromise the ability to perform testing. Testing requires a dedicated collection tube.

Testing Schedule:

Weekly

Analytic Time:

7 working days

Reference Range:

Direct detection of G-T tranversion at position 1849 of the Janus kinase 2 locus, giving rise to a Valine-to-Phenylalanine substitution at position 617 (JAK2 V617F mutation).

Comments:

This test is useful for diagnosis of myeloproliferative isorders: Polycythemia Vera, Essential Thrombocythemia and Chronic Idiopathic Myelofibrosis.

Methodology:

Allele specific Polymerase Chain Reaction followed by fluorescence capillary electrophoresis

Sample Processing:

Label transport tube with two patient identifiers, date and time of collection.
Patient's age and sex is required on requisition for processing.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Lavender tube is sent as whole blood, no processing necessary.
Do Not Centrifuge.

Sample Storage:

Room temperature for up to 24 hours, then refrigerate the whole blood if it is necessary to be held overnight, or weekends, or holidays.

Transport Instructions:

Place requisition into outside pocket of bag.
Recommend express mail or equivalent if not on courier service.

CPT Code:

83909, 83900, 83890, 83912