BRAF Mutation Analysis
| Order Form: | Molecular Oncology Requisition |
Specimen:
Formalin fixed, paraffin embedded tissue block and 10 unstained 5-10 μM slides at 20-25°C and an H&E slide of the tissue
Minimum:
Tumor cells more than 50% of the total tissue and greater than
10mm2 in surface area on the block.
Rejection Criteria:
Specimens fixed in B5 fixative or that have been decalcified will not
be accepted. Tumor specimens containing less than 50% tumor cells or
are less than 10mm2 in area may be unacceptable.
Testing
Schedule:
Weekly
Analytic Time:
7-10 working days
Reference Range:
Negative
Comments:
Order Code: BRAF
To aid in the diagnosis of papillary thyroid carcinoma.
To aid in the diagnosis of papillary thyroid carcinoma.
Methodology:
Polymerase Chain Reaction (PCR) followed by Sequencing
Sample
Processing:
Formalin-fixed, paraffin embedded tissue should be submitted.
Sample
Storage:
Ambient or Room Temperature.
Transport
Instructions:
Place specimen into zip-lock type bag, seal bag.
Recommend express mail or equivalent if not on courier service.
Ship at ambient temperature.
Place requisition into outside pocket of bag.
Protect block or slides from excessive heat and ship in cooled container during summer months.
Recommend express mail or equivalent if not on courier service.
Ship at ambient temperature.
Place requisition into outside pocket of bag.
Protect block or slides from excessive heat and ship in cooled container during summer months.
CPT Code:
83907, 83904, 83898, 83890, 83912