Specimen:

Plasma

Collection Medium:

Alternate
Collection Media:

Lavender top tube (EDTA), Light Green top tube (Lithium Heparin), Red 
top tube, Green top tube (Na Heparin), Pink top tube (EDTA sprayed)

Minimum:

Preferred Minimum: 1.0 mL plasma
Absolute Minimum: 0.3 mL plasma

Rejection Criteria:

Grossly hemolyzed or lipemic specimens.

Analytic Time:

4 working days

Reference Range:

Deficiency:  Less than 20 ng/mL
Insufficiency:  20-29 ng/mL
Optimum Level:  30-80 ng/mL
Possible Toxicity:  Greater than 80 ng/mL

Interpretive Data:

This is the appropriate screening test for routine assessment of 
vitamin D nutritional status.  This assay accurately quantifies the sum 
of vitamin D3, 25-Hydroxy and vitamin D2, 25-Hydroxy.  Optimum 
25-hydroxy vitamin D concentration is 35 ng/mL or higher.  For workup 
of hypercalcemia, vitamin D status in renal failure patients, and 
certain malignancies, "Vitamin D (1,25-dihydroxy)" may be indicated.

Methodology:

Chemiluminescent Immunoassay

Instructions:

Contact University of Iowa Diagnostic Laboratories 
1-866-844-2522 (toll free) 
319-384-7213 (Fax)

CPT Code:

82306