IGH Gene Clonality by PCR with Interpretation
Label Mnemonic: | IGH, IGHGENE |
Epic code: | LAB8283 for Blood, LAB2471 for Bone Marrow and Tissue |
Order form: | Molecular Oncology Requisition Comprehensive Hematopathology Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
CPT code: | 81261 |
Specimen(s):
Blood, Bone Marrow and Tissue
Collection Medium:
or | ||
Pink top tube 6 mL (K2-EDTA) | Yellow top tube 8.5 mL (ACD solution A) |
Minimum:
5 mL blood in a pink top or yellow top, 1 mL bone marrow aspirate
in a pink top or yellow top, 3 cu mm of fresh frozen tissue or
Formalin-fixed, paraffin embedded tissue, body fluids in a pink top
or yellow top or lymph node aspirates in RPMI.
Testing
Schedule:
Weekly
Turn Around
Time:
7 working days
Reference Range:
None
Comments:
DNA extracted from blood, bone marrow mononuclear cells or tissue is
examined for rearrangement of immunoglobin heavy chain genes.
Test
Limitations:
Specimens fixed in B5 fixative are unsuitable for testing.
Methodology:
Multiplex PCR followed by Fluorescence Capillary Electrophoresis
Sample
Processing:
Label transport tube with two patient identifiers, date and time of collection.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen source is required on requisition for processing.
Tissue specimen should be frozen.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen source is required on requisition for processing.
Tissue specimen should be frozen.
Sample
Storage:
Refrigerate blood specimens overnight if shipping cannot occur the same day of collection. Do not freeze specimens.
Tissue Specimen - Freeze immediately and store frozen or send Formalin-fixed paraffin embedded blocks.
Tissue Specimen - Freeze immediately and store frozen or send Formalin-fixed paraffin embedded blocks.
Transport
Instructions:
Ship refrigerated.
Frozen Tissue - Ship frozen on dry ice. Ship express mail with overnight delivery. Formalin-fixed paraffin embedded blocks ship at room temperature.
Frozen Tissue - Ship frozen on dry ice. Ship express mail with overnight delivery. Formalin-fixed paraffin embedded blocks ship at room temperature.
CPT Code:
81261