IGH Gene Clonality by PCR, Various

IGH Gene Clonality by PCR

Order Code:	BCELLPCR
Order Form:	Molecular Oncology Requisition

Specimen:

Various

Collection Medium:

	or
Pink top tube	or	Yellow top tube (ACD solution A)

Minimum:

5 mL blood in a pink top or yellow top, 1 mL bone marrow aspirate in a pink top or yellow top, 3 cu mm of fresh frozen tissue or Formalin-fixed, paraffin embedded tissue, body fluids in a pink top or yellow top or lymph node aspirates in RPMI.

Testing Schedule:

Weekly

Analytic Time:

7 working days

Reference Range:

None

Comments:

DNA extracted from blood, bone marrow mononuclear cells or tissue is examined for rearrangement of immunoglobin heavy chain genes.

Test Limitations:

Specimens fixed in B5 fixative are unsuitable for testing.

Methodology:

Multiplex PCR followed by Fluorescence Capillary Electrophoresis

Sample Processing:

Label transport tube with two patient identifiers, date and time of collection.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen source is required on requisition for processing.
Tissue specimen should be frozen.
Lavender tube is sent as whole blood, no processing necessary.

Sample Storage:

Blood/Bone Marrow - Room temperature or ambient storage.
Tissue Specimen - Freeze immediately and store frozen or send Formalin-fixed paraffin embedded blocks.

Transport Instructions:

Blood - Do not freeze, ship at ambient temperature. Ship express mail with overnight delivery.
Frozen Tissue - Ship frozen on dry ice. Ship express mail with overnight delivery. Formalin-fixed paraffin embedded blocks ship at ambient temperature.

CPT Code:

83900, 83901(x4), 83907, 83909(x3), 83912