Fluorescence In-Situ Hybridization (FISH-Aneuploidy Screening)
Label Mnemonic: ANEUP
Order form: Constitutional Genetics Pre and Post-Natal Requisition
Supply order: Supply Order Form
Billing: Billing Policies
CPT code: 88271 x5, 88275 x5, 88291
Specimen(s):
Paraffin Embedded Tumor Tissue or Peripheral Blood (Newborn or Cord, and Others)
Minimum:
4 sections cut at 2 microns and an accompanying H&E side from the tissue block with the most villi.

2 cc of infants venous blood collected in a green-top Vacutainer® with sodium heparin. Invert tube to mix well. Label the tube with patient name and medical record number. DO NOT FREEZE OR CENTRIFUGE.
Testing Schedule:
Specimens accepted in the lab Monday-Friday, 0800-1700.
Turn Around Time:
Preliminary results are given for STAT blood cases within 48 hours. Suitable for interphase and metaphase analysis. Allow 2-5 days for final results. Preliminary results are not provided for paraffin embedded tumor tissue samples.
Reference Range:
Male:  X and Y probe signals          Female:  two X probe signals
The reference range for autosomes varies for each probe, call lab for 
information.
Methodology:
Fluorescence In-Situ Hybridization (FISH)
Instructions:
Submit specimen to laboratory as soon as possible after collection. Provide details of clinical information and family history (if applicable). If a specimen is collected over the weekend, please call (319) 356-1616 and ask the operator to page the cytogenetics on-call staff at pager #5525.

For billing and/or shipping questions, contact University of Iowa Diagnostic Laboratories at 1-866-844-2522 (toll free) or 319-384-7213 (Fax).
Sample Processing:
Do Not Centrifuge.
Maintain sterility and forward promptly to lab.
Sample Storage:
Specimen cannot be frozen.
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Ship at room temperature.
CPT Code:
88271 x5, 88275 x5, 88291