Fluorescence In-Situ Hybridization (FISH-Microdeletion)

Order form:	Constitutional Genetics Pre and Post-Natal Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	88271 x # of probes 88273 x # of probes, 88291

Specimen(s):

Peripheral Blood, Fibroblasts, Other Tissue (fibroblasts, and OT need to establish cultures first, not going to meet 5-7 day TAT)

Collection Medium:

Green top tube 10 mL (Na Heparin) Hospital Stores #907693

Minimum:

5-10 cc adult, 2 cc infants of venous blood collected in a green-top Vacutainer^® with sodium heparin. Invert tube to mix well. Label the tube with patient name and medical record number. DO NOT FREEZE OR CENTRIFUGE.

Testing Schedule:

Specimens accepted in the lab Monday-Friday, 0800-1700.

Turn Around Time:

Allow 5-7 days for final results.

Reference Range:

Varies for each probe (set). Unique sequence probes labeled with fluorochrome. Contact lab for details.

Comments:

Epic code: CYT54

This test can be used as an adjunct to chromosomal analysis for detection of submicroscopic/cryptic rearrangements in certain congenital disorders. Suitable for metaphase analysis only.

Probes currently available include:
1p36
Angelman syndrome
Cri-du-chat
Deletion 22q syndromes
Langergied
Miller-Dieker syndrome
Prader-Willi syndrome
Smith-Magenis syndrome
SRY(Yp11.3)
STS[del(Xp22.3)]
Williams syndrome
Wolf-Hirschhorn syndrome

Shivanand R. Patil Cytogenetics & Molecular Laboratory Website

Methodology:

Fluorescence In-Situ Hybridization (FISH)

Instructions:

Provide details of clinical information.  If a specimen is 
collected over the weekend, please call (319) 356-1616 and ask the 
operator to page the cytogenetics on-call staff at pager #5525.  

For billing and/or shipping questions, contact University of Iowa 
Diagnostic Laboratories at 1-866-844-2522 (toll free) or 319-384-7213 
(Fax).

Sample Processing:

Do Not Centrifuge.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.

Sample Storage:

Specimen cannot be frozen.

Transport Instructions:

Place specimen into zip-lock type bag, seal bag.
Ship at room temperature.

CPT Code:

88271 x # of probes
88273 x # of probes, 88291