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University of Iowa Diagnostic Laboratories (UIDL) Test Directory 319-384-7212 (local) 1-866-844-2522 (toll free) |
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| Parainfluenza Antigen Detection by DFA | |
| Order Code: C PFLU
Order Form: A-1a Clinical Microbiology Laboratory Requisition |
Specimen: |
Nasopharyngeal Swab/Aspirate/Wash, Tracheal Aspirate, Bronchoalveolar Lavage, Bronchial Wash | ||
Collection Medium: |
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Minimum: |
Collect nasopharyngeal swab in viral transport medium (33595). Alternatively, collect tracheal aspirate, bronchoalveolar lavage, bronchial wash, or nasopharyngeal wash/aspirate in sterile, leak-proof container. | ||
Rejection Criteria: |
Sputum or dry swab. | ||
Testing Schedule: |
0700-1630, 7 days a week, including holidays. | ||
Analytic Time: |
Results for respiratory antigen detection by DFA are provided the same day if specimen is received in the Microbiology lab by 1430. Respiratory virus culture will only be set up if the DFA is negative. Culture results are available in 48 h. | ||
Comments: |
Screening test for Parainfluenza 1, 2 and 3. Sensitivity of the DFA is dependent on adequacy of the specimen. Culture will be ordered to confirm negative DFA results. See "Viral Culture" for additional specimen collection instructions. | ||
Methodology: |
Direct Fluorescent Antibody (DFA) | ||
Sample Processing: |
Indicate specimen/fluid type on requisition. Label transport tube with two patient identifiers, date and time of collection. Maintain sterility and forward promptly to lab. Specimen should be collected and packaged as close to shipping time as possible. Submit in leakproof container. Specimen source is required on requisition for processing. | ||
Sample Storage: |
Refrigerate. | ||
Transport Instructions: |
Coolant packs up to 8 hours. Place coolant packs into styrofoam container. Place requisition into outside pocket of bag. Recommend express mail or equivalent if not on courier service. | ||
CPT Code: |
87279 | ||
See also: Viral Culture |
Updated: 11/03/2009