Endomysial IgA Antibody Screen with Reflex Titer and Interpretation
Order Code: EMAIGA
Order Form: Immunopathology Requisition
Specimen:
Serum
Collection Medium:
Red top tube
Minimum:
Adult - 5 mL; red top tube
Pediatric - 2 mL; red top tube
Testing Schedule:
Bi-weekly (Mon and Thurs) - Batch analysis performed twice weekly on Mondays and Thursdays excluding university holidays.
Turn Around Time:
1 week
Reference Range:
< 1:5 Titer Endomysial IgA antibody is detected in 70-76% of patients with dermatitis herpetiformis and 90+% of patients with celiac disease who are on a gluten-containing diet. Occurence of endomysial IgA antibody decreases significantly on gluten-free diets. Note: Selective IgA deficiencies affect 3-5% of celiac and dermatitis herpetiformis patients and this condition will cause a "false negative" test. If IgA deficiency is known, please note requisition and IgG anti-EMA test will be done instead of IgA.
Comments:
Include relevant clinical information and consultation request.
Methodology:
Indirect Immunofluorescence
Sample Processing:
Centrifuge at 3500 RPM for 5 minutes.
Aliquot serum into labeled container and cap.
Sample Storage:
Refrigerate serum 2-8°C.
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at ambient temperature.
CPT Code:
86255 Endomysial Antibody IgA screen 86255-26 Endomysial Antibody IgA screen interpretation 86256 Endomysial Antibody IgA titer 86256-26 Endomysial Antibody IgA titer interpretation