Total T, B and NK Cell Lymphocyte Quantitation
Order Form: Flow Cytometry Requisition
Specimen:
Peripheral Blood
Minimum:
Adult and Pediatric: Peripheral Blood, 10 mL; yellow top. Must also submit lavender top (5 mL), simultaneously drawn, for wbc/diff.
Testing Schedule:
0800-1630 Monday through Friday.
Analytic Time:
2 days
Reference Range:
The pathologist will provide an interpretative report. Adult reference ranges for peripheral blood by whole blood lysis method using flow cytometry: Absolute Counts B cells (CD19) 6-22% 53-726/mm3 T cells (CD3) 65-85% 569-2804/mm3 T Cells (CD4) 34-62% 298-2045/mm3 T cells (CD8) 14-42% 122-1386/mm3 T helper/induce (CD4+/CD3+) 30-62% 263-2045/mm3 T cytotoxic/suppressor (CD8+/CD3+) 13-37% 114-1221/mm3 NK cells (CD16+/CD56+/CD3-) 5-31% 44-1023/mm3 NK Cells (CD56) 5-29% 44-957/mm3 CD4/CD8 ratio 0.9-3.6 Age specific pediatric reference ranges will be provided with the interpretive report.
Comments:
Maintain sample at room temperature; do not incubate or refrigerate. Please print, complete and submit the Advance Beneficiary Notice (ABN) along with the Flow Cytometry Requisition before shipping the specimen.
Test Limitations:
Specimens with absolute lymphocyte counts of <100/mm3 will not be tested.
Methodology:
Flow Cytometry-Whole Blood Lysis
Sample Processing:
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen should be collected and packaged as close to shipping time as possible.
Sample Storage:
Ambient or Room Temperature.
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at ambient temperature.
Recommend express mail or equivalent if not on courier service.
CPT Code:
CPT Codes Technical: 88184 x1 and 88185 x7
CPT Codes Professional: 88187 - 26
 
See Additional Information:
Flow Cytometry Consultation Overview