Lymphoma Panel
| Order Form: | Flow Cytometry Requisition |
Specimen:
Peripheral Blood, Bone Marrow, Fresh Lymph Node, Fine Needle Aspirate, or Body Fluids NOS
Collection Medium:
 |
| Yellow top tube (ACD solution A) |
Alternate
Collection Media:
Green top tube 4 mL (Na Heparin)
Minimum:
Adult and Pediatric; Peripheral Blood: 10 mL; yellow top tube.
Adult and Pediatric; Bone Marrow or Body Fluid; 2-5 mL; Green top tube
(Na Heparin) or heparinized syringe (use approximately 1000 units).
Fluids and Tissue: Dispense sample into vial of RPMI-160 tissue culture
media. RPMI-1640 minimizes cell death in such specimens, especially if
processing cannot be done within 48 hours of collection.
Testing
Schedule:
0800-1630 Monday through Friday.
Analytic Time:
2 days
Reference Range:
Antibodies routinely included are: (CD2, CD3, CD4, CD5, CD8, CD10,
CD14, CD19, CD20, CD23, CD45, Kappa, Lambda
If clinical or morphologic features suggest a "T" or "NK" lymphocyte
disorder, then the following additional antibody combinations are
performed: CD3/CD4/CD8, CD7/CD5/HLA-DR, CD25/CD2/CD3, CD16/CD56/CD19,
CD57/CD8/CD3, TCR alpha-beta/delta-gamma/CD3.
The pathologist will provide an interpretative report.
Comments:
Please print, complete and submit the Advance Beneficiary
Notice (ABN) along with the Flow Cytometry Requisition before
shipping the specimen.
Please state the clinical question to be answered on the requisition.
Maintain the specimen at room temperature; do not refrigerate or
incubate.
Methodology:
Flow Cytometry-Whole Blood Lysis
Instructions:
Keep at room temperature. Do not refrigerate. Deliver specimen
immediately to Specimen Control to ensure leukocyte viability.
Sample
Processing:
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen should be collected and packaged as close to shipping time as possible.
Specimen should be collected and packaged as close to shipping time as possible.
Sample
Storage:
Ambient or Room Temperature.
Transport
Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at ambient temperature.
Recommend express mail or equivalent if not on courier service.
Place requisition into outside pocket of bag.
Ship at ambient temperature.
Recommend express mail or equivalent if not on courier service.
CPT Code:
Technical: 88184 x1 and 88185 x12
Professional: 88188 - 26
See Additional Information:
Flow Cytometry Consultation Overview
Flow Cytometry Consultation Overview