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University of Iowa Diagnostic Laboratories (UIDL) Test Directory 319-384-7212 (local) 1-866-844-2522 (toll free) |
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| Celiac Disease/Dermatitis Herpetiformis (DH) Panel |
Order Form: Immunopathology Requisition |
Specimen: |
Serum |
Alternate Collection Media: |
Red top tube |
Minimum: |
Adult - 5 ml; red top tube Pediatric - 2 ml; red top tube |
Testing Schedule: |
0800-1630 Monday through Friday. |
Analytic Time: |
1 week |
Reference Range: |
Endomysial(DH)Antibody IgA: <1:5 Titer
Gliadin IgA and IgG: Negative: <20 units
Weak Positive: 20-30 units
Moderate to Stong Positive: >30 units
Tissue Transglutaminase: < 20 units |
Comments: |
Endomysial IgA antibody is detected in 70-76% of patients with
dermatitis herpetiformis and 90+% of patients with celiac
disease who are on a gluten-containing diet. Occurence of endomysial
IgA antibody decreases significantly on gluten-free diets. Note:
Selective IgA deficiencies affect 3-5% of celiac and dermatitis
herpetiformis patients and this condition will cause a "false negative"
test. If IgA deficiency is known, please note on the requisition and
IgG anti-EMA test will be done instead of IgA.
Selective IgA deficiency affects 3-5% of celiac and dermatitis
herpetiformis patients.
CPT codes: 86255 Endomysial Antibody IgA screen
86255-26 Endomysial Antibody IgA screen interpretation
86256 Endomysial Antibody IgA titer
86256-26 Endomysial Antibody IgA titer interpretation
83520 Gliadin IgA Antibody
83520 Gliadin IgG Antibody
83520 Tissue Transglutaminase Antibody |
Methodology: |
Endomysial Antibody IgA: Indirect Immunofluorescence Gliadin IgA, Gliadin IgG, and Tissue Transglutaminase: Enzyme-Linked Immunosorbent Assay (ELISA) |
Sample Processing: |
Centrifuge at 3500 RPM for 5 minutes. Aliquot serum into labeled container and cap. |
Sample Storage: |
Refrigerate serum 2-8 degree C. |
Transport Instructions: |
Place specimen into zip-lock type bag, seal bag. Place requisition into outside pocket of bag. Ship at ambient temperature. |
See also: Deamidated Gliadin Peptide IgA Antibody, Serum Deamidated Gliadin Peptide IgG Antibody, Serum Tissue Transglutaminase, Serum |
Updated: 07/18/2005