Specimen:

Serum

Collection Medium:

Minimum:

Adult minimum: 5 mL red top tube
Pediatric minimum: 2 mL; red top tube

Testing
Schedule:

Weekly
Test performed on Thursdays.

Analytic Time:

1 week

Reference Range:

IgG class antibodies:  Negative: Less than or equal to 20 SGU
                       Positive: >20 SGU

IgM class antibodies:  Negative: Less than or equal to 20 SMU
                       Positive: >20 SMU

Results are reported out in standard IgG or IgM units (SGU) and (SMU)

Methodology:

Enzyme-Linked Immunosorbent Assay (ELISA)

Sample
Processing:

Centrifuge at 3500 RPM for 5 minutes.
Aliquot serum into labeled container and cap.

Sample
Storage:

Refrigerate serum 2-8 degree C.

Transport
Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at ambient temperature.

CPT Code:

86146 Beta 2 Glycoprotein Antibody, IgG
86146 Beta 2 Glycoprotein Antibody, IgM

See Additional Information:
Antiphospholipid Syndrome (APS): Laboratory Evaluation