Vancomycin Drug Level
Label Mnemonic: VANC
Epic code: LAB41
Order form: Laboratory Requisition
Supply order: Supply Order Form
Billing: Billing Policies
CPT code: 80202
Specimen(s):
Plasma
Collection Medium:
Plasma Separator Tube 4.5 mL
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood in light green top tube or ONE Microtainer®.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
peak 30-40 μg/mL; (30-60 minutes after I.V. infusion)
trough  5-10 μg/mL; (not more than 30 minutes before next dose)

Critical value: >30 μg/mL
Comments:
Assay methodology updated to Roche Diagnostics Vancomycin III assay on 8/1/17. Critical value threshold changed on 12/5/17.
Test Limitations:
Hemolysis: No significant interference up to an H index of 1000 (approximate hemoglobin concentration: 1000 mg/dL).

Lipemia: No significant interference up to an L index of 1000 (approximate triglycerides concentration: 1000 mg/dL). Note there is poor correlation between turbidity and triglyceride concentration.

Icterus: No significant interference up to an I index of 60 (approximate conjugated and unconjugated bilirubin concentration: 60 mg/dL).

No significant interference from total protein up to 12 g/dL or rheumatoid factors up to 1200 IU/mL.

As with any assay employing mouse antibodies, the possibility exists for interference by human anti-mouse antibodies (HAMA) in the sample, which could cause falsely lowered results.
Methodology:
Kinetic Interaction of Microparticles in Solution (KIMS)
Sample Processing:
Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Sample Storage:
Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
Transport Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
80202
 
See Additional Information:
Chemistry Critical Lab Values