Specimen:

Plasma

Collection Medium:

Alternate
Collection Media:

Call laboratory for additional acceptable specimen collection 
containers.

Minimum:

2 ml; plasma separator tube top or 2 microtubes.

Rejection Criteria:

Red top tube is not acceptable.

Testing
Schedule:

24 hrs/day, 7 days a week, including holidays.

Analytic Time:

2 hours (upon receipt in laboratory)

Reference Range:

Is less than or equal to 0.10 ng/ml

Comments:

Please print, complete and submit the Advance Beneficiary Notice 
(ABN) along with the Laboratory Requisition before shipping the 
specimen.

Troponin T method instituted 3/13/00 at 0700.

Test
Limitations:

The assay is unaffected by icterus (bilirubin is less than 27 mg/dl), 
hemolysis (Hb is less than 0.1 g/dl), lipemia (Intralipid is less than 
1500 mg/dl) and biotin is less than 50 ng/ml (criterion: recovery 
within plus or minus 10% of initial value).

In patients receiving therapy with high biotin doses (i.e. is greater 
than 5 mg/day) no sample should be taken until at least 8 hours after 
the last biotin administration.

No interference was observed from rheumatoid factor up to 2000 U/ml.

There is no high-dose hook effect for troponin T concentrations up to 
400 ng/ml.

In vitro tests were performed on 50 commonly used pharmaceuticals. No 
interference with the assay was found.

Plasma samples collected using tubes containing oxalate/fluoride, 
revealed sample-dependent low troponin T values when compared to 
results obtained on serum samples. Therefore, do not use 
oxalate/fluoride plasma samples for the assay.

As with all tests containing monoclonal mouse antibodies, erroneous 
findings may be obtained from samples taken from patients who have been 
treated with monoclonal mouse antibodies or have received them for 
diagnostic purposes.

In rare cases, interference due to extremely high titers of antibodies 
to streptavidin can occur.

Troponin T reagent contains additives which minimize these effects.

Extremely high titers of antibodies to ruthenium can occur in isolated 
cases and cause interference.

For diagnostic purposes, the Troponin T findings should always be 
assessed in conjunction with the patient's medical history, clinical 
examination and other findings.

Methodology:

Electrochemiluminescence Imunoassay

Sample
Processing:

Centrifuge at 3000 RPM for 10 minutes.
Aliquot serum into labeled container and cap.

Sample
Storage:

Refrigerate.

Transport
Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

84484