University of Iowa
Diagnostic Laboratories
(UIDL) Test Directory

319-384-7212 (local)
1-866-844-2522 (toll free)


Triglycerides
Order Code: TRIG
Order Form: Laboratory Requisition
Specimen:
Plasma
Collection Medium:
Plasma separator tube
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection 
containers.
Minimum:
2 mls whole blood in light green top tube or 1 microtube for pediatric 
patients
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
2 hours (upon receipt in laboratory)
Reference Range:
Normal:  < 150 mg/dL
Borderline High:  150-199 mg/dL
High:  200-499 mg/dL
Very High:  > or = 500 mg/dL

To convert triglyceride values to mm/L, divide by 88.6.
Comments:
Patient must have fasted for 12 hours.

Please print, complete and submit the Advance Beneficiary Notice 
(ABN) along with the Laboratory Requisition before shipping the 
specimen.
Test
Limitations:
Serum/Plasma
Icterus: Significant negative interference at an I index of greater 
than 25 and greater than 15 (approximate conjugated and unconjugated 
bilirubin concentration: 25 and 15 mg/dL).

Hemolysis: No significant interference up to an H index of 400 
(approximate hemoglobin concentration: 400 mg/dL).

Lipemia (Intralipid): The L index correlates with sample turbidity and 
not to triglycerides level. Serum samples having extremely elevated 
triglycerides (greater than 3000 mg/dL) are underestimated by the 
Triglycerides/GB methodology and could produce a normal result on an 
elevated sample.*  Prior to being tested for triglycerides, dilute 
grossly lipemic sera 1 part serum to 4 parts 0.9% NaCl and multiply the 
results by 5. For the 911, 912 and 917 analyzers, run with decreased 
samplevolume. Not all samples having this level of triglycerides are 
grossly lipemic.

*Shephard MDS, Whiting MJ. Clin Chem. 1990;36(2):325-329.
Methodology:
Enzymatic
Sample
Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Sample
Storage:
Refrigerate.
Transport
Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship ambient or refrigerate.
Instructions:
Patient must fast 10-12 hours prior to drawing.
CPT Code:
84478
 
See Additional Information:
Fasting Specimen Requirements

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Updated: 08/31/2007