Tobramycin
Order Code: TOB
Order Form: Laboratory Requisition
Specimen:
Plasma
Collection Medium:
Plasma Separator Tube
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood in light green top or ONE microtainer.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
1 hour (upon receipt in laboratory)
Reference Range:
Peak 6-10 mcg/mL; (45-75 min. after I.M. dose, 15-30 min. after I.V. dose) Trough <2 mcg/mL; (Not more than 30 min. before next dose) Critical value: Peak >10 mcg/mL
Comments:
Phlebotomy team does not draw timed tobramycin specimens. Clinical staff must draw accurately timed peak and trough specimens.
Test Limitations:
Criterion: recovery plus or minus 0.24 ug/mL of initial value at concentrations less than 2.4 ug/mL or plus or minus 10% of initial value at concentrations greater than 2.4 ug/mL. Icterus: No significant interference from bilirubin up to an I index of 30 (approximate bilirubin concentration: 30 mg/dL). Hemolysis: No significant interference from hemoglobin up to an H index of 800 (approximate hemoglobin concentration: 800 mg/dL). Lipemia (Intralipid): No significant interference up to an L index of 750. There is poor correlation between turbidity and triglycerides concentration. No significant interference from total protein up to 12 g/dL.
Methodology:
EIA (Enzymatic ImmunoAssay)
Sample Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Label pre- or post- specimens clearly.
Sample Storage:
Refrigerate.
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Place dry ice on top of specimen in styrofoam container.
CPT Code:
80200
 
See Additional Information:
Chemistry Critical Lab Values