Specimen:

Plasma

Collection Medium:

Alternate
Collection Media:

Call laboratory for additional acceptable specimen collection 
containers.

Minimum:

2 mLs whole blood in light green top or 0.6 mL microtube

Testing
Schedule:

24 hrs/day, 7 days a week, including holidays.

Analytic Time:

2 hours (upon receipt in laboratory)

Reference Range:

Peak 6-10 mcg/mL; (45-75 min. after I.M. dose, 15-30 min. after I.V. 
dose)
Trough <2 mcg/mL; (Not more than 30 min. before next dose)

Critical value: Peak   >10 mcg/mL

Comments:

Phlebotomy team does not draw timed tobramycin specimens.  Clinical 
staff must draw accurately timed peak and trough specimens.

Test
Limitations:

Criterion: recovery plus or minus 0.24 ug/mL of initial value at 
concentrations less than 2.4 ug/mL or plus or minus 10% of initial 
value at concentrations greater than 2.4 ug/mL.

Icterus: No significant interference from bilirubin up to an I index of 
60 (approximate bilirubin concentration: 60 mg/dL).

Hemolysis: No significant interference from hemoglobin up to an H index 
of 1000 (approximate hemoglobin concentration: 1000 mg/dl).

Lipemia (Intralipid): No significant interference up to an L index of 
1000 (approximate triglycerides concentration: 2000 mg/dl). There is 
poor correlation between turbidity and triglycerides concentration.

No significant interference from total protein up to 13.2 g/dl, IgA up 
to 2700 mg/dl, IgG up to 4900 mg/dl, IgM up to 900 mg/dl and rheumatoid 
factor up to 1200 IU/ml.

Methodology:

EIA (Enzymatic ImmunoAssay)

Sample
Processing:

Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Label pre- or post- specimens clearly.

Sample
Storage:

Refrigerate.

Transport
Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Place dry ice on top of specimen in styrofoam container.

CPT Code:

80200

See Additional Information:
Chemistry Critical Lab Values