Tobramycin Drug Level

Label Mnemonic:	TOB
Epic code:	LAB36
Order form:	Laboratory Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	80200

Specimen(s):

Plasma

Collection Medium:

Plasma Separator Tube 4.5 mL

Alternate Collection Media:

Call laboratory for additional acceptable specimen collection containers.

Minimum:

3 mL whole blood in light green top or ONE Microtainer^®.

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Turn Around Time:

1 hour (upon receipt in laboratory)

Reference Range:

Peak 6-10 μg/mL; (45-75 min. after I.M. dose, 15-30 min. 
after I.V. dose)
Trough <2 μg/mL; (Not more than 30 min. before next dose)

Critical value: Peak   >10 μg/mL

Comments:

Phlebotomy team does not draw timed tobramycin specimens. Clinical staff must draw accurately timed peak and trough specimens.

Test Limitations:

Icterus: No significant interference from bilirubin up to an I 
index of 30.

Hemolysis: No significant interference from hemoglobin up to an H 
index 
of 800.

Lipemia (Intralipid): No significant interference up to an L index of 
750. There is poor correlation between turbidity and triglycerides 
concentration.

No significant interference from total protein up to 12 g/dL.

Methodology:

Homogenous Enzyme Immunoassay

Sample Processing:

Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.

Sample Storage:

Refrigerate.

Transport Instructions:

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

80200

See Additional Information:
Chemistry Critical Lab Values