Thyroid Stimulating Hormone (TSH), Reflexive
| Order Code: | TSHR |
| Order Form: | Laboratory Requisition |
Specimen:
Plasma
Collection Medium:
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| Plasma Separator Tube |
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood in light green top or THREE microtainers.
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
1 hour (upon receipt in laboratory)
Reference Range:
AGE MALES AND FEMALES
1 - 11 months 0.8-6.3 mIU/mL
1 - 5 years 0.7-5.9 mIU/mL
> 5 years Same as adult values
Adults 0.27-4.20 mIU/mL
Comments:
If TSH is outside the adult reference range, a Free T4 is automatically
analyzed, at an additional charge to the patient.
Please print, complete and submit the Advance Beneficiary
Notice (ABN) along with the Laboratory Requisition before shipping
the specimen.
New analytical immunoassay with different reference range instituted
4/24/00 at 1000.
Test
Limitations:
The assay is unaffected by icterus (bilirubin is less than 41 mg/dL),
hemolysis (Hb is less than 1000 mg/dL), lipemia (Intralipid is less
than
1500 mg/dL) and biotin is less than 25 ng/mL (criterion: recovery
within plus or minus 10% of initial value).
In patients receiving therapy with high biotin doses (i.e. is greater
than 5 mg/day) no sample should be taken until at least 8 hours after
the last biotin administration.
No interference was observed from rheumatoid factor (up to 3250 U/mL)
and samples from dialysis patients.
There is no high-dose hook effect for TSH concentrations up to 1000
μIU/mL.
In vitro tests were performed on 26 commonly used pharmaceuticals. No
interference with the assay was found.
As with all tests containing monoclonal mouse antibodies, erroneous
findings may be obtained from samples taken from patients who have been
treated with monoclonal mouse antibodies or have received them for
diagnostic purposes.
In rare cases, interference due to extremely high titers of antibodies
to ruthenium can occur. TSH reagent contains additives which minimize
these effects.
Extremely high titers of antibodies to streptavidin can occur in
isolated cases and cause interference.
For diagnostic purposes, the TSH findings should always be assessed in
conjunction with the patient's medical history, clinical examination
and other findings.
Methodology:
Electrochemiluminescence Immunoassay
Sample
Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Aliquot plasma into labeled container and cap.
Sample
Storage:
Room temperature or refrigerate if stored overnight, weekends or holidays.
Transport
Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship ambient or refrigerate.
Place requisition into outside pocket of bag.
Ship ambient or refrigerate.
CPT Code:
84443
