Specimen:

Plasma

Collection Medium:

Alternate
Collection Media:

Call laboratory for additional acceptable specimen collection 
containers.

Minimum:

5 ml; whole blood in light green top tube

Testing
Schedule:

24 hrs/day, 7 days a week, including holidays.

Analytic Time:

2 hours (upon receipt in laboratory)

Reference Range:

<14 IU/ml

Test
Limitations:

Criterion: Recovery within plus or minus 10% of initial value.

Icterus: No significant interference up to an I index of 60 
(approximateconjugated and unconjugated bilirubin concentration: 60 
mg/dl or 1026 umol/l).

Hemolysis: No significant interference up to an H index of 1000 
(approximate hemoglobin concentration: 1000 mg/dl or 621 umol/l).

Lipemia (Intralipid): No significant interference up to an L index of 
500.

There is poor correlation between turbidity and triglycerides 
concentration.

No high-dose hook effect up to RF activities of 6000 IU/ml.

Thirty one commonly used pharmaceuticals were tested in vitro. No 
interference with the assay was found.

Reference:
Glick MR, Ryder KW, Jackson SA. Graphical Comparisons of Interferences 
in Clinical Chemistry Instrumentation. Clin Chem 1986;32:470-474.

Methodology:

Immunoturbidimetric Assay

Sample
Processing:

Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.

Sample
Storage:

Refrigerate.

Transport
Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

86431