Reticulocyte Count (Automated)
Order Code: ARET
Order Form: Laboratory Requisition
Specimen:
Whole Blood
Collection Medium:
Lavender top tube 3 mL (EDTA)
Minimum:
Full draw; 3.0 mL lavender top; (or fingerstick)
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
1 hour (upon receipt in laboratory)
Reference Range:
Age Absolute Reticulocyte Range 1 - 3 Days* 77 - 283 K/mm3 4 - 30 Days* 14 - 159 K/mm3 31 - 60 Days* 28 - 201 K/mm3 60 Days - Adult 12 - 130 K/mm3 *Full Term Infant
Comments:
A reticulocyte count can usually be added to any CBC sample <24 hours old.
Methodology:
Flow Cytometry Reticulocyte counts are performed using flow cytometry method. The RNA/DNA in the Reticulocytes is stained and the stained cells are then detected and enumerated on the basis of a two-dimensional distribution of the forward scattered light and lateral fluorescent light.
Sample Processing:
Submit whole blood in original container.
Do Not Centrifuge.
Sample Storage:
Refrigerate.
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
85045