Reticulocyte Count (Automated)
Label Mnemonic: ARET
Epic code: LAB296
Order form: Laboratory Requisition
Supply order: Supply Order Form
Billing: Billing Policies
CPT code: 85045
Specimen(s):
Whole Blood
Collection Medium:
Lavender top tube 3 mL (EDTA)
Minimum:
Full draw; 3.0 mL lavender top; (or fingerstick-purple top microtainer®)
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
Age                     Absolute Reticulocyte Range
      1 - 3 Days*            77 - 283 K/mm3
      4 - 30 Days*           14 - 159 K/mm3
     31 - 60 Days*           28 - 201 K/mm3
60 Days - Adult              12 - 130 K/mm3
*Full Term Infant

% Reticulocytes:  0.4 - 2.1%
Comments:
Refer to BD Microtainer® Tubes product sheet for detailed sample collection instructions.

A reticulocyte count can usually be added to any CBC sample <24 hours old.
Methodology:
Flow Cytometry
Reticulocyte counts are performed using flow cytometry method.  The 
RNA/DNA in the Reticulocytes is stained and the stained cells are then 
detected and enumerated on the basis of a two-dimensional distribution 
of the forward scattered light and lateral fluorescent light.
Sample Processing:
Lavender tube is sent as whole blood, no processing necessary.
Sample Storage:
Refrigerate, 2-8°C.
Transport Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
85045