Anti-Glomerular Basement Membrane Antibody
Order Code: AGBM
Order Form: Immunopathology Requisition
Specimen:
Serum
Collection Medium:
Red top tube
Minimum:
Adult - 5 mL; red top tube Pediatric - 2 mL; red top tube
Testing Schedule:
Bi-weekly (Tues and Fri) - Batch analysis performed twice weekly on Tuesdays and Fridays excluding university holidays.
Analytic Time:
3 days
Reference Range:
Negative: <=20 ELISA units Weak positive: 21-30 ELISA units Positive: >30 ELISA units
Comments:
The results will be obtained with the INOVA QUANTA Lite™ ELISA (a semi-quantitative assay). Assay values obtained with different manufacturers' methods may not be used interchangeably. The magnitude of the reported antibody levels do not correlate with the antibody titer in a linear fashion.

Because the assay is semi-quantitative, it is intended as a diagnostic test only. It is not intended for use as a tool for frequent monitoring of antibody levels, nor is it designed to evaluate acute response to therapy such as immunosuppressant medication or plasmapheresis.
Methodology:
Enzyme-Linked Immunosorbent Assay Test (ELISA)
Sample Processing:
Centrifuge at 3500 RPM for 5 minutes.
Aliquot serum into labeled container and cap.
Sample Storage:
Refrigerate serum 2-8°C.
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at ambient temperature.
CPT Code:
83520