Glomerular Basement Membrane Antibodies, IgG
Label Mnemonic: | GBM |
Epic code: | LAB1255 |
Order form: | Laboratory Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
CPT code: | 83520 |
Specimen(s):
Serum
Collection Medium:
Red top tube 5 mL (Clot Activator) |
Minimum:
Adult - 5 mL; red top tube Pediatric - 2 mL; red top tube
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around
Time:
3 hours (upon receipt in laboratory)
Reference Range:
Negative: < 0.4 antibody index (AI)
Equivocal: 0.4-0.9
Positive: 1.0 AI or greater
Equivocal: 0.4-0.9
Positive: 1.0 AI or greater
Interpretive Data:
Glomerular basement membrane (GBM) antibodies are used for evaluating
with rapid onset renal failure or pulmonary hemorrhage and as an aid in
the diagnosis of Goodpasture syndrome in conjunction with clinical
history and physical examination. Positive results for GBM antibodies
are consistent with Goodpasture syndrome. GBM antibodies are highly
specific for Goodpasture syndrome. The sensitivity of this test is
approximately 87% in untreated patients with systemic disease.
Comments:
Assay methodology and reference ranges changed February 5, 2013.
References:
Pusey CD. Anti-glomerular basement membrane disease. Kidney Int 2003; 64:1535-1550.
References:
Pusey CD. Anti-glomerular basement membrane disease. Kidney Int 2003; 64:1535-1550.
Methodology:
Multiplex flow immunoassay
Sample
Processing:
Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells.
Separate plasma or serum into labeled container and cap.
Separate plasma or serum into labeled container and cap.
Sample
Storage:
Refrigerate.
Transport
Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
83520