Specimen:

Serum

Collection Medium:

Minimum:

Adult - 5 mL; red top tube
Pediatric - 2 mL; red top tube

Testing
Schedule:

Bi-weekly (Tues and Fri) - Batch analysis performed 
twice weekly on Tuesdays and Fridays excluding 
university holidays.

Analytic Time:

3 days

Reference Range:

<20 negative

Comments:

Confirmatory assay for Anti-Neutrophil Cytoplasmic Antibodies, will 
only be performed when ANCA testing is positive or inconclusive.

Methodology:

Enzyme-Linked Immunosorbent Assay (ELISA)

Sample
Processing:

Centrifuge at 3500 RPM for 5 minutes.
Aliquot serum into labeled container and cap.

Sample
Storage:

Refrigerate serum 2-8 degree C.

Transport
Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at ambient temperature.

CPT Code:

83520