Specimen:

Plasma

Collection Medium:

Alternate
Collection Media:

Call laboratory for additional acceptable specimen collection 
containers.

Minimum:

2 mls whole blood in light green top tube or two 0.6 microtubes

Testing
Schedule:

24 hrs/day, 7 days a week, including holidays.

Analytic Time:

2 hours (upon receipt in laboratory)

Reference Range:

MALES                     0.2 -  1.4 ng/ml
FEMALES - menstrating:
Follicular Phase          0.2 -  1.5 ng/ml
Ovulation Phase           0.8 -  3.0 ng/ml
Luteal Phase              1.7 - 27.0 ng/ml

POSTMENOPAUSAL FEMALES   <0.2 -  0.8 ng/ml

Comments:

New analytical immunoassay with different reference ranges instituted 
3/13/00 at 0700.

Test
Limitations:

The assay is unaffected by icterus = 54(bilirubin ditaurate is less 
than 54 mg/dl), hemolysis = 1000 (Hb is less than 1.0 g/dl),  lipemia = 
720 (Intralipid is less than 720 mg/dl) and biotin is less than 20 
ng/ml. (criterion: recovery within plus or minus 10% of initial value).

In patients receiving therapy with high biotin doses (i.e. is greater 
than 5 mg/day) no sample should be taken until at least 8 hours after 
the last biotin administration.

No interference was observed from rheumatoid factors up to a 
concentration of 2000 U/ml.

In vitro tests were performed on 18 commonly used pharmaceuticals. Of 
these, only phenylbutazone at therapeutic dosage levels showed 
interference with the assay (progesterone values depressed).

As with all tests containing monoclonal antibodies, erroneous findings 
may be obtained from samples taken from patients who have been treated 
with monoclonal mouse antibodies or who have received them for 
diagnostic purposes.

In rare cases, interference due to extremely high titers of antibodies 
to ruthenium can occur.

Progesterone II contains additives which minimize these effects.

Extremely high titers of antibodies to streptavidin can occur in 
isolated cases and cause interference.

For diagnostic purposes, the Progesterone II findings should always be 
assessed in conjunction with the patient's medical history, clinical 
examination and other findings.

Methodology:

Electrochemiluminescence Immunoassay

Sample
Processing:

Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.

Sample
Storage:

Refrigerate.

Transport
Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

84144