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University of Iowa Diagnostic Laboratories (UIDL) Test Directory 319-384-7212 (local) 1-866-844-2522 (toll free) |
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| Phosphatase, Alkaline | Order Code: ALP
Order Form: Laboratory Requisition |
Specimen: |
Plasma | ||
Collection Medium: |
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Alternate Collection Media: |
Call laboratory for additional acceptable specimen collection containers. | ||
Minimum: |
2 ml whole blood in light green top tube or 1 microtube for pediatric patients | ||
Testing Schedule: |
24 hrs/day, 7 days a week, including holidays. | ||
Analytic Time: |
2 hours (upon receipt in laboratory) | ||
Reference Range: |
Males: 40-129 u/l; females: 35-104 u/l (higher in children and adolescents) | ||
Test Limitations: |
Criterion: Recovery within plus or minus 10% of initial values. Icterus: No significant interference up to an I Index of 70 (conjugated bilirubin) and an I Index of 70 (unconjugated bilirubin) corresponding to approximately 70 mg/dl conjugated and 70 mg/dl unconjugated bilirubin. Hemolysis: No significant interference up to an H index of 500 (approximate hemoglobin concentration: 500 mg/dl). Lipemia (Intralipid): No significant interference up to an L index of 2000. There is poor correlation between turbidity and triglycerides concentration. Reference Glick MR, Ryder KW, Jackson SA. Graphical Comparisons of Interferences in Clinical Chemistry Instrumentation. Clin Chem 1986;32:470-474. | ||
Methodology: |
Spectrophotometric | ||
Sample Processing: |
Centrifuge at 3000 RPM for 10 minutes. Aliquot plasma into labeled container and cap. | ||
Sample Storage: |
Refrigerate. | ||
Transport Instructions: |
Place requisition into outside pocket of bag. Place specimen into zip-lock type bag, seal bag. Transport in cooler with refrigerated coolant packs. | ||
Instructions: |
Add-on testing permitted only within 24 hours of draw time. | ||
CPT Code: |
84075 |
Updated: 06/30/2004