University of Iowa
Diagnostic Laboratories
(UIDL) Test Directory

319-384-7212 (local)
1-866-844-2522 (toll free)


Phosphatase, Alkaline Order Code: ALP
Order Form: Laboratory Requisition
Specimen:
Plasma
Collection Medium:
Plasma separator tube
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection 
containers.
Minimum:
2 ml whole blood in light green top tube or 1 microtube for pediatric 
patients
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
2 hours (upon receipt in laboratory)
Reference Range:
Males: 40-129 u/l; females: 35-104 u/l
(higher in children and adolescents)
Test
Limitations:
Criterion: Recovery within plus or minus 10% of initial values.

Icterus: No significant interference up to an I Index of 70 (conjugated 
bilirubin) and an I Index of 70 (unconjugated bilirubin) corresponding 
to approximately 70 mg/dl conjugated and 70 mg/dl unconjugated 
bilirubin.

Hemolysis: No significant interference up to an H index of 500 
(approximate hemoglobin concentration: 500 mg/dl).

Lipemia (Intralipid): No significant interference up to an L index of 
2000. There is poor correlation between turbidity and triglycerides 
concentration.

Reference
Glick MR, Ryder KW, Jackson SA. Graphical Comparisons of Interferences 
in Clinical Chemistry Instrumentation. Clin Chem 1986;32:470-474.
Methodology:
Spectrophotometric
Sample
Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Sample
Storage:
Refrigerate.
Transport
Instructions:
Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Transport in cooler with refrigerated coolant packs.
Instructions:
Add-on testing permitted only within 24 hours of draw time.
CPT Code:
84075

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Updated: 06/30/2004