University of Iowa
Diagnostic Laboratories
(UIDL) Test Directory

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Phosphatase, Alkaline
Order Code: ALP
Order Form: Laboratory Requisition
Specimen:
Plasma
Collection Medium:
Plasma separator tube
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection 
containers.
Minimum:
2 ml whole blood in light green top tube or 1 microtube for pediatric 
patients
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
2 hours (upon receipt in laboratory)
Reference Range:
       AGE                MALES         FEMALES
      1 - 30 days      75-316 U/L     48-406 U/L
31 days - 1 year       82-383 U/L    124-341 U/L
      1 - 3 years     104-345 U/L    108-317 U/L
      4 - 6 years      93-309 U/L     96-297 U/L
      7 - 9 years      86-315 U/L     69-325 U/L
     10 - 12 years     42-362 U/L     51-332 U/L
     13 - 15 years     74-390 U/L     50-162 U/L
     16 - 18 years     52-171 U/L     47-119 U/L
         Adults        40-129 U/L     35-104 U/L
Test
Limitations:
Criterion: Recovery within plus or minus 10% of initial values.

Icterus: No significant interference up to an I Index of 70 (conjugated 
bilirubin) and an I Index of 70 (unconjugated bilirubin) corresponding 
to approximately 70 mg/dl conjugated and 70 mg/dl unconjugated 
bilirubin.

Hemolysis: No significant interference up to an H index of 500 
(approximate hemoglobin concentration: 500 mg/dl).

Lipemia (Intralipid): No significant interference up to an L index of 
2000. There is poor correlation between turbidity and triglycerides 
concentration.

Reference
Glick MR, Ryder KW, Jackson SA. Graphical Comparisons of Interferences 
in Clinical Chemistry Instrumentation. Clin Chem 1986;32:470-474.
Methodology:
Spectrophotometric
Sample
Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Sample
Storage:
Refrigerate.
Transport
Instructions:
Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Transport in cooler with refrigerated coolant packs.
Instructions:
Add-on testing permitted only within 24 hours of draw time.
CPT Code:
84075

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Updated: 04/14/2009