University of Iowa
Diagnostic Laboratories
(UIDL) Test Directory

319-384-7212 (local)
1-866-844-2522 (toll free)


Pentobarbital (Nembutal) (As a Therapeutic Agent) Order Code: PENT
Order Form: Laboratory Requisition
Specimen:
Plasma
Collection Medium:
Plasma separator tube
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection 
containers.
Minimum:
5 mL whole blood from light green top tube or two light green top 
microtubes for pediatric patients.
Testing
Schedule:
0700-1530 Monday through Friday; 0600-1200 Saturday, Sunday and 
holidays; sample must be received by 1200 Saturday, Sunday and holidays 
for same day service.
For additional services contact Clinical Pathology Resident on-call at 
#3404.
Analytic Time:
4 hours (upon receipt in laboratory)
Reference Range:
Used in treatment of increased intracranial pressure with
concentrations of 25-40 mcg/ml.  Mean half-life of 26.5 hours.
Ref: Cpt 20, p. 67 (1976).
Comments:
Testing Schedule
0700-1530 Monday through Friday.
0600-1200 Saturday, Sunday and holidays.
Sample must be received by 1200 Saturday, Sunday and holidays for same 
day service.
For additional services contact Clinical
Pathology Resident on-call at #3404.
Methodology:
FPIA
Sample
Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Label transport tube with two patient identifiers, date and time of 
collection.
Sample
Storage:
Refrigerate.
Transport
Instructions:
Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Transport in cooler with refrigerated coolant packs.
Instructions:
Contact UIDL Client Services:
1-866-844-2522
CPT Code:
82205

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Updated: 07/14/2008