Parathyroid Hormone (Intact)
| Label Mnemonic: | PTHP |
| Epic code: | LAB108 |
| Order form: | Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 83970 |
Specimen(s):
Plasma
Collection Medium:
 |
| Pink top tube 6 mL (K2-EDTA) |
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
6 mL whole blood in pink top tube
Rejection Criteria:
Grossly hemolyzed or lipemic specimens
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around
Time:
1 hour (upon receipt in laboratory)
Reference Range:
15-65 pg/mL
Comments:
This assay may be significantly impacted by high-dose biotin (>5 mg
dose) taken within previous 12 hours. High concentrations of biotin
may lead to falsely decreased results. These concentrations may be
found in patients taking over-the-counter supplements with biotin
content much higher than nutritional requirements for biotin.
Specimens should not be collected until at least 12 hours after the
last dose.
Test
Limitations:
Hemolysis: No significant interference up to an H index of 250
(approximate hemoglobin concentration: 250 mg/dL).
Lipemia: No significant interference up to an L index of 1500 (approximate triglycerides concentration: 3000 mg/dL). Note there is poor correlation between turbidity and triglyceride concentration.
Icterus: No significant interference up to an I index of 65 (approximate conjugated and unconjugated bilirubin concentration: 65 mg/dL).
Lipemia: No significant interference up to an L index of 1500 (approximate triglycerides concentration: 3000 mg/dL). Note there is poor correlation between turbidity and triglyceride concentration.
Icterus: No significant interference up to an I index of 65 (approximate conjugated and unconjugated bilirubin concentration: 65 mg/dL).
Methodology:
Electrochemiluminescence Immunoassay
Instructions:
Spun plasma is stable for 3 days at 2-8°C and 6 months at
-20°C.
Sample
Processing:
Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection.
Separate plasma into screw-capped plastic vial.
Do not send primary tube if all plasma has been transferred to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Separate plasma into screw-capped plastic vial.
Do not send primary tube if all plasma has been transferred to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Sample
Storage:
Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
Transport
Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
83970
See Additional Information:
Biotin Interference with Immunoassays
Biotin Interference with Immunoassays