Specimen:

Plasma

Collection Medium:

Alternate
Collection Media:

Call laboratory for additional acceptable specimen collection 
containers.

Minimum:

2 ml in lithium heparin or 2 microtubes

Testing
Schedule:

24 hrs/day, 7 days a week, including holidays.

Analytic Time:

1 hour (upon receipt in laboratory)

Reference Range:

Males: 28-72 ng/ml
Females: 25-58 ng/ml

Test
Limitations:

The assay is unaffected by icterus (bilirubin is less than 65mg/dl), 
hemolysis (Hb is less than 1400 mg/dl), lipemia (less than 2200) and 
biotin is less than 50 ng/ml.

In patients receiving therapy with high biotin doses (greater than 5 
mg/day) no sample should be taken until at least 8 hours after last 
biotin given.

No interference was observed from rheumatoid factor up to 1500 U/ml.

There is no high-dose hook affect for myoglobin concentrations up to 
30,000 mg/ml.

In vitro tests were performed on 50 commonly used pharmaceuticals.  No 
interference with the assay was found.

As with all tests containing monoclonal mouse antibodies, erroneous 
findings may be obtained from samples taken from patients who have been 
treated with monoclonal mouse antibodies or have received them for 
diagnostic purposes.

In rare cases, interference due to extremely high titers of antibodies 
to Ruthenium can occur.  The Roche myoglobin reagent contains additives 
which minimize these effects.

Extremely high titers of antibodies to Streptavidin can occur in 
isolated cases and cause interference.

For diagnostic purposes, the Myoglobin findings should always be 
assesed in conjunction with ther patient's medical history, clinical 
examination and other findings.

Methodology:

Electrochemiluminescence Immunoassay

Sample
Processing:

Centrifuge immediately after drawing specimen.
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into screw-capped plastic vial.

Sample
Storage:

Frozen up to two weeks.

Transport
Instructions:

Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Place dry ice on top of specimen in styrofoam container.
Dry Ice up to 24 hours.

CPT Code:

83874