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University of Iowa Diagnostic Laboratories (UIDL) Test Directory 319-384-7212 (local) 1-866-844-2522 (toll free) |
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| Mumps, IgG Antibody Detection | |
| Order Code: MUMP
Order Form: A-1a Clinical Microbiology Laboratory Requisition |
Specimen: |
Serum | ||
Collection Medium: |
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Minimum: |
2 ml; red top tube (no additive) | ||
Testing Schedule: |
Test performed twice weekly. | ||
Analytic Time: |
3 days | ||
Reference Range: |
Negative | ||
Comments: |
Test performed twice weekly. | ||
Test Limitations: |
This test is a qualitative enzyme-linked fluorescent immunoassay (ELFA) for the detection of IgG antibodies to mumps virus to provide a means of determining the immune status of individuals. Mumps is a paramyxovirus that causes an acute generalized infection primarily in children and adolescents (1). Mumps is usually a self-limited, benign infection with one-third of infected individuals remaining asymptomatic, however, complications (meningitis, encephalitis, epididymo-orchitis, oophoritis, et al) may occur (1). A mumps vaccine used in the U.S. since 1967 is credited with a >99% drop in the annual number of cases documented in 1996 (2). A negative mumps IgG test result indicates possible susceptibility to mumps infection. If a patient has an equivocal result, a new specimen will be requested for repeat testing. A positive test result indicates prior exposure to mumps (usually by vaccination) or a convalescent stage of infection. A false positive result is possible due to cross-reactions with other paramyxoviruses (i.e., parainfluenza viruses) (3). Positive results in patients that have received blood products within the past 3 months may not indicate the patient's true immune status. References 1) Baum SG and N Litman. 2000. "Mumps virus." In G.M. Mandell., J.E. Bennett, & R. Dolin. (Eds.) Mandell, Douglas, and Bennett's principles and practice of infectious diseases. 5th ed. (pp. 1776- 1781). Philadelphia, PA: Churchill Livingstone. 2) Centers for Disease Control and Prevention. 1997. Status report on the childhood immunization initiative: reported cases of selected vaccine-preventable diseases-United States, 1996. MMWR 46:665-671. 3) Harmsen T, et al. 1992. Comparison of a neutralization enzyme immunoassay for evaluation of immune status of children vaccinated for mumps. J Clin Microbiol 30:2139-2144. | ||
Methodology: |
Enzyme-Linked Fluorescent Immunoassay (ELFA) | ||
Sample Processing: |
Label transport tube with two patient identifiers, date and time of collection. Aliquot serum into labeled container and cap. Centrifuge at 3000 RPM for 10 minutes. Centrifuge within one hour of draw time. | ||
Sample Storage: |
Refrigerate serum 2-8 degree C. | ||
Transport Instructions: |
Use refrigerated coolant packs. Place requisition into outside pocket of bag. Place specimen into zip-lock type bag, seal bag. | ||
CPT Code: |
86735 | ||
See Additional Information: Microbiology Specimen Collection and Transport |
Updated: 10/06/2009