University of Iowa
Diagnostic Laboratories
(UIDL) Test Directory

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Luteinizing Hormone (LH)
Order Code: LH
Order Form: Laboratory Requisition
Specimen:
Plasma
Collection Medium:
Plasma separator tube
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection 
containers.
Minimum:
2 ml whole blood in light green top tube or two 0.6 microtubes
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
2 hours (upon receipt in laboratory)
Reference Range:
FEMALES - menstruating:
  Follicular phase      2.4 - 12.6 mIU/ml
  Ovulation phase      14.0 - 95.6 mIU/ml
  Luteal phase          1.0 - 11.4 mIU/ml
  Postmenopause         7.7 - 58.5 mIU/ml
MALES:                  1.7 -  8.6 mIU/ml

    AGE            MALES            FEMALES
Prepubertal      <0.5 mIU/mL      <0.5 mIU/mL
Tanner II     0.2-2.8 mIU/mL   0.1-4.1 mIU/mL
Tanner III    1.2-3.9 mIU/mL   0.2-9.2 mIU/mL
Tanner IV     0.9-4.4 mIU/mL   0.7-8.6 mIU/mL
Tanner V      1.8-5.3 mIU/mL   0.5-7.3 mIU/mL
Comments:
New immunoassay method instituted 3/21/00 at 0900.
Test
Limitations:
The assay is unaffected by icterus (bilirubin is less than 66 mg/dl), 
hemolysis (Hb is less than 1 g/dl), lipemia (Intralipid is less than 
1900 mg/dl) and biotin is less than 50 ng/ml (criterion: recovery 
within plus or minus 10% of initial value).

In patients receiving therapy with high biotin doses (i.e. is greater 
than 5 mg/day) no sample should be taken until at least 8 hours after 
the last biotin administration.

No interference was observed from rheumatoid factors up to a 
concentration of 1500 U/ml.

No high dose hook effect at LH concentrations of up to 1150 mIU/ml In 
vitro tests were performed on 17 commonly used pharmaceuticals. No 
interference with the assay was found.

Samples of neonates have not been tested with LH assay.

As with all tests containing monoclonal antibodies, erroneous findings 
may be obtained from samples taken from patients who have been treated 
with monoclonal mouse antibodies or who have received them for 
diagnostic purposes.

In rare cases interference due to extremely high titers of antibodies 
to streptavidin and ruthenium can occur.

LH reagent contains additives which minimize these effects.

For diagnostic purposes, the LH findings should always be assessed in 
conjunction with the patient's medical history, clinical examination 
and other findings.
Methodology:
Electrochemiluminescence Immunoassay
Sample
Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Sample
Storage:
Refrigerate.
Transport
Instructions:
Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
83002

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Updated: 04/14/2009