Chronic Lymphocytic Leukemia
| Order Form: | Flow Cytometry Requisition |
Specimen:
Various
Collection Medium:
 |
| Yellow top tube (ACD solution A) |
Alternate
Collection Media:
Green top tube 4 mL (Na Heparin), Lavender top tube 3 mL (EDTA)
Minimum:
Adult
Peripheral Blood: 10 mL; Bone Marrow: 2-4 mL yellow top tube (ACD
solution A)
Pediatric
Peripheral Blood: 2-5 mL; Bone Marrow: 2-5 mL lavender top tube
(EDTA) or green top tube (Na Heparin).
Fluids and Tissue: Dispense sample into vial of RPMI-1640 tissue
culture media.
Testing
Schedule:
0800-1630 Monday through Friday.
Analytic Time:
2 days
Reference Range:
Antibodies routinely included are: CD2, CD3, CD5, CD10, CD11c, CD14,
CD19, CD20, CD22, CD23, CD25, CD38, CD45, CD103, CD138, FMC7, Heavy
chains, Kappa and Lambda. Antibodies will also identify cases of
Prolymphocytic and Hairy Cell Leukemia.
If clinical or morphologic features suggest a "T" or "NK" lymphocyte
disorder, then the following additional antibody combinations are
performed: CD3/CD4/CD8, CD7/CD5/HLA-DR, CD14/CD45, CD25/CD2/CD3,
CD16/CD56/CD19, CD57/CD8/CD3, TCR alpha-beta/delta-gamma/CD3.
The pathologist will provide an interpretative report.
Comments:
Please state the clinical question to be answered on the requisition.
Maintain the specimen at room temperature; do not refrigerate or
incubate.
Please print, complete and submit the Advance Beneficiary
Notice (ABN) along with the Flow Cytometry Requisition before
shipping the specimen.
Methodology:
Flow Cytometry-Whole Blood Lysis
Sample
Processing:
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen should be collected and packaged as close to shipping time as possible.
Specimen should be collected and packaged as close to shipping time as possible.
Sample
Storage:
Ambient or Room Temperature.
Transport
Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at ambient temperature.
Recommend express mail or equivalent if not on courier service.
Place requisition into outside pocket of bag.
Ship at ambient temperature.
Recommend express mail or equivalent if not on courier service.
CPT Code:
88184, 88185(x20) technical; 88189 professional; T cell or NK cell
disorder: 88184, 88185(x15) technical; 88188 professional
See Additional Information:
Flow Cytometry Consultation Overview
Flow Cytometry Consultation Overview