Lactate Dehydrogenase (LD)
Order Code: LD
Order Form: Laboratory Requisition
Specimen:
Plasma
Collection Medium:
Plasma Separator Tube
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood in light green top tube or ONE microtainer for pediatric patients.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
1 hour (upon receipt in laboratory)
Reference Range:
Adult Males 135-225 u/l Adult Females 135-214 u/l Pediatric Reference Ranges Male Female 0-31 days 125-735 u/l 145-765 u/l 31 days-1 year 170-450 190-420 1 year-4 years 155-345 165-395 4 years-7 years 155-345 135-345 7 years-10 years 145-300 140-280 10 years-13 years 120-290 120-260 13 years-16 years 120-290 100-275 16 years-19 years 105-235 105-230
Comments:
Avoid hemolysis. False elevations may occur in specimens which are not processed promptly to separate serum from RBC's.
Test Limitations:
Criterion: recovery plus or minus 10% of initial values Plasma Icterus: No significant interference up to an I index of 60 (approximate conjugated and unconjugated bilirubin concentration: 60 mg/dL). Hemolysis: RBC contamination elevates results; interference if H index is greater than or equal to 40. Lipemia (Intralipid): No significant interference up to an L index of 1000. There is poor correlation between turbidity and triglycerides concentration. Highly lipemic samples may cause ABS! flags.
Methodology:
UV Assay
Sample Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Sample Storage:
Refrigerate.
Transport Instructions:
Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Ship at ambient temperature.
CPT Code:
83615
 
See also:
Lactate Dehydrogenase-Other, Body Fluid (e.g., surgical drain, pericardial, peritoneal, peritoneal dialysis, pleural, or synovial)