Specimen:

Plasma

Collection Medium:

Alternate
Collection Media:

Call laboratory for additional acceptable specimen collection 
containers.

Minimum:

2 mls whole blood in light green top tube or 2 microtubes for pediatric 
patients

Testing
Schedule:

24 hrs/day, 7 days a week, including holidays.

Analytic Time:

2 hours (upon receipt in laboratory)

Reference Range:

Adult Males   135-225 u/l
Adult Females 135-214 u/l

Pediatric Reference Ranges
                   Male          Female
0-31 days          125-735 u/l   145-765 u/l
31 days-1 year     170-450       190-420
1 year-4 years     155-345       165-395
4 years-7 years    155-345       135-345
7 years-10 years   145-300       140-280
10 years-13 years  120-290       120-260
13 years-16 years  120-290       100-275
16 years-19 years  105-235       105-230

Comments:

Avoid hemolysis.  False elevations may occur in specimens which are not 
processed promptly to separate serum from RBC's.

Test
Limitations:

Criterion: recovery plus or minus 10% of initial values

Plasma
Icterus: No significant interference up to an I index of 60 
(approximate conjugated and unconjugated bilirubin concentration: 60 
mg/dl).

Hemolysis: RBC contamination elevates results; interference if H index 
is greater than or equal to 20.

Lipemia (Intralipid):  No significant interference up to an L index of 
1000.

There is poor correlation between turbidity and triglycerides 
concentration. Highly lipemic samples may cause ABS! flags.

Methodology:

Enzymatic

Sample
Processing:

Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.

Sample
Storage:

Refrigerate.

Transport
Instructions:

Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Ship at ambient temperature.

CPT Code:

83615