Lymphocyte Subsets
Order Form: Flow Cytometry Requisition
Specimen:
Peripheral Blood
Collection Medium:
Yellow top tube (ACD solution A)
Alternate Collection Media:
Lavender top tube 3 mL (EDTA)
Minimum:
Adult: 10 mL whole blood in a yellow top tube (ACD solution A)
Pediatric: 2 mL whole blood in a lavender top tube (EDTA)

For absolute quantitative results, a CBC with Automated Differential must also be ordered.
Testing Schedule:
0800-1630 Monday through Friday.
Analytic Time:
2 days
Reference Range:
The pathologist will provide an interpretative report. Antibodies routinely included are: CD3, CD4, CD8, CD14, CD16/56, CD19, CD20 and CD45. Adult reference ranges for peripheral blood by whole blood lysis method using flow cytometry: Absolute Counts B cells (CD19) 6-22% 53-726/mm3 T cells (CD3) 65-85% 569-2804/mm3 T cells (CD4) 34-62% 298-2045/mm3 T cells (CD8) 14-42% 122-1386/mm3 T helper/induce (CD4+/CD3+) 30-62% 263-2045/mm3 T cytotoxic/suppressor (CD8+/CD3+) 13-37% 114-1221/mm3 NK cells (CD16+/CD56+/CD3-) 5-31% 44-1023/mm3 NK cells (CD56) 5-29% 44-957/mm3 CD4/CD8 ratio 0.9-3.6 Age specific pediatric reference ranges will be provided with the interpretive report.
Comments:
Specimens with absolute lymphocyte counts of <100/mm3 will not be tested.
Test Limitations:
Specimens with absolute lymphocyte counts of <100/mm3 will not be tested.
Methodology:
Flow Cytometry-Whole Blood Lysis
Sample Processing:
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen should be collected and packaged as close to shipping time as possible.
Sample Storage:
Ambient or Room Temperature.
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at ambient temperature.
Recommend express mail or equivalent if not on courier service.
CPT Code:
88184 x1, 88185 x7 Technical, 88187 Professional
 
See Additional Information:
Flow Cytometry Consultation Overview