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University of Iowa Diagnostic Laboratories (UIDL) Test Directory 319-384-7212 (local) 1-866-844-2522 (toll free) |
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| Lymphocyte Subsets | |
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Order Form: Flow Cytometry Requisition |
Specimen: |
Peripheral Blood | ||
Collection Medium: |
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Alternate Collection Media: |
Lavender top tube (EDTA) | ||
Minimum: |
Adult: 10 mL whole blood in a yellow top tube (ACD solution A) Pediatric: 2 mL whole blood in a lavender top tube (EDTA) For absolute quantitative results, a CBC with Automated Differential must also be ordered. | ||
Testing Schedule: |
0800-1630 Monday through Friday. | ||
Analytic Time: |
2 days | ||
Reference Range: |
The pathologist will provide an interpretative report. Antibodies
routinely included are: CD3, CD4, CD8, CD14, CD16/56, CD19, CD20 and
CD45.
Adult reference ranges for peripheral blood by whole blood lysis method
using flow cytometry:
Absolute Counts
B cells (CD19) 6-22% 53-726/mm3
T cells (CD3) 65-85% 569-2804/mm3
T cells (CD4) 34-62% 298-2045/mm3
T cells (CD8) 14-42% 122-1386/mm3
T helper/induce (CD4+/CD3+) 30-62% 263-2045/mm3
T cytotoxic/suppressor (CD8+/CD3+) 13-37% 114-1221/mm3
NK cells (CD16+/CD56+/CD3-) 5-31% 44-1023/mm3
NK cells (CD56) 5-29% 44-957/mm3
CD4/CD8 ratio 0.9-3.6
Age specific pediatric reference ranges will be provided with the
interpretive report. | ||
Comments: |
Specimens with absolute lymphocyte counts of <100/mm3 will not be tested. | ||
Test Limitations: |
Specimens with absolute lymphocyte counts of <100/mm3 will not be tested. | ||
Methodology: |
Flow Cytometry-Whole Blood Lysis | ||
Sample Processing: |
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results. Specimen should be collected and packaged as close to shipping time as possible. | ||
Sample Storage: |
Ambient or Room Temperature. | ||
Transport Instructions: |
Place specimen into zip-lock type bag, seal bag. Place requisition into outside pocket of bag. Ship at ambient temperature. Recommend express mail or equivalent if not on courier service. | ||
CPT Code: |
88184 x1, 88185 x7 Technical, 88187 Professional | ||
See Additional Information: Flow Cytometry Consultation Overview |
Updated: 03/03/2009