Specimen:

Plasma

Collection Medium:

Alternate
Collection Media:

Call laboratory for additional acceptable specimen collection 
containers.

Minimum:

2 ml whole blood from light green top tube

Rejection Criteria:

Specimens received in Sodium Citrate tubes

Testing
Schedule:

24 hrs/day, 7 days a week, including holidays.

Analytic Time:

2 hours (upon receipt in laboratory)

Reference Range:

40-230 mg/dl

Test
Limitations:

Criterion: Recovery within plus or minus 10% of initial values.

Icterus: No significant interference up to an I index of 60 
(approximate conjugated and unconjugated bilirubin concentration: 60 
mg/dl).

Hemolysis: No significant interference up to an H index of 1000. 
(approximate hemoglobin concentration: 1000 mg/dl)

Lipemia (Intralipid): No significant interference up to an L index of 
3000.  There is poor correlation between turbidity and triglycerides 
concentration.

There is no cross-reaction between IgM and IgA or IgG under assay 
conditions.

Sera with ill-defined characteristics should be subject to protein 
electrophoresis to identify a possible antigen excess (e.g. 
gammopathy).

A high dose hook effect may occur at IgM concentrations of greater than 
23,000 mg/dl (230 g/l).

Methodology:

Immunoturbidimetric

Sample
Processing:

Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.

Sample
Storage:

Refrigerate.

Transport
Instructions:

Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

82784