Immunoglobulin A, Individual Quant, Plasma

Immunoglobulin A, Individual Quant

Order Code:	IGA
Order Form:	Laboratory Requisition

Specimen:

Plasma

Collection Medium:

Plasma Separator Tube

Alternate Collection Media:

Call laboratory for additional acceptable specimen collection containers.

Minimum:

3 mL whole blood from light green top tube or ONE microtainer

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Analytic Time:

1 hour (upon receipt in laboratory)

Reference Range:

70-400 mg/dL Children and Juveniles 0-1 year 0-83 mg/dL 1-3 years 20-100 mg/dL 4-6 years 27-195 mg/dL 7-9 years 34-305 mg/dL 10-11 years 53-204 mg/dL 12-13 years 58-358 mg/dL 14-15 years 47-249 mg/dL 16-19 years 61-348 mg/dL 20-adult 70-400 mg/dL

Test Limitations:

Criterion: Recovery within plus or minus 10% of initial value. Icterus: No significant interference up to an I index of 60 (approximate conjugated and unconjugated bilirubin concentration: 60 mg/dL). Hemolysis: No significant interference up to an H index of 1000 (approximate hemoglobin concentration: 1000 mg/dL). Lipemia (Intralipid): No significant interference up to an L index of 3000). There is poor correlation between turbidity and triglycerides concentration. There is no cross-reaction between IgA and IgG or IgM under the assay conditions. Plasma with ill-defined characteristics should be subject to protein electrophoresis to identify a possible antigen excess (e.g. gammopathy). A high-dose hook effect may occur at concentrations above 12,000 mg/dL (120 g/l).

Methodology:

Immunoturbidimetric

Sample Processing:

Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.

Sample Storage:

Refrigerate.

Transport Instructions:

Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

82784