University of Iowa
Diagnostic Laboratories
(UIDL) Test Directory

319-384-7212 (local)
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Immunoglobulin A, Individual Quant
Order Code: IGA
Order Form: Laboratory Requisition
Specimen:
Plasma
Collection Medium:
Plasma separator tube
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection 
containers.
Minimum:
2 ml whole blood from light green top tube
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
2 hours (upon receipt in laboratory)
Reference Range:
70-400 mg/dl
Test
Limitations:
Criterion: Recovery within plus or minus 10% of initial value.

Icterus: No significant interference up to an I index of 60 
(approximate conjugated and unconjugated bilirubin concentration: 60 
mg/dl).

Hemolysis: No significant interference up to an H index of 1000 
(approximate hemoglobin concentration: 1000 mg/dl).

Lipemia (Intralipid): No significant interference up to an L index of 
3000). There is poor correlation between turbidity and triglycerides 
concentration.

There is no cross-reaction between IgA and IgG or IgM under the assay 
conditions.

Plasma with ill-defined characteristics should be subject to protein 
electrophoresis to identify a possible antigen excess (e.g. 
gammopathy).

A high-dose hook effect may occur at concentrations above 12,000 mg/dl 
(120 g/l).
Methodology:
Immunoturbidimetric
Sample
Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Sample
Storage:
Refrigerate.
Transport
Instructions:
Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
82784

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Updated: 01/06/2006