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University of Iowa Diagnostic Laboratories (UIDL) Test Directory 319-384-7212 (local) 1-866-844-2522 (toll free) |
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| Immunoglobulin A, Individual Quant | |
| Order Code: IGA
Order Form: Laboratory Requisition |
Specimen: |
Plasma | ||
Collection Medium: |
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Alternate Collection Media: |
Call laboratory for additional acceptable specimen collection containers. | ||
Minimum: |
2 ml whole blood from light green top tube | ||
Testing Schedule: |
24 hrs/day, 7 days a week, including holidays. | ||
Analytic Time: |
2 hours (upon receipt in laboratory) | ||
Reference Range: |
70-400 mg/dl | ||
Test Limitations: |
Criterion: Recovery within plus or minus 10% of initial value. Icterus: No significant interference up to an I index of 60 (approximate conjugated and unconjugated bilirubin concentration: 60 mg/dl). Hemolysis: No significant interference up to an H index of 1000 (approximate hemoglobin concentration: 1000 mg/dl). Lipemia (Intralipid): No significant interference up to an L index of 3000). There is poor correlation between turbidity and triglycerides concentration. There is no cross-reaction between IgA and IgG or IgM under the assay conditions. Plasma with ill-defined characteristics should be subject to protein electrophoresis to identify a possible antigen excess (e.g. gammopathy). A high-dose hook effect may occur at concentrations above 12,000 mg/dl (120 g/l). | ||
Methodology: |
Immunoturbidimetric | ||
Sample Processing: |
Centrifuge at 3000 RPM for 10 minutes. Aliquot plasma into labeled container and cap. | ||
Sample Storage: |
Refrigerate. | ||
Transport Instructions: |
Place requisition into outside pocket of bag. Place specimen into zip-lock type bag, seal bag. Transport in cooler with refrigerated coolant packs. | ||
CPT Code: |
82784 |
Updated: 01/06/2006