University of Iowa
Diagnostic Laboratories
(UIDL) Test Directory

319-384-7212 (local)
1-866-844-2522 (toll free)


Immunofixation Electrophoresis Order Code: SIFE
Order Form: Immunopathology Requisition
Specimen:
Serum
Collection Medium:
Red top tube
Minimum:
Adult- 2 ml; red top tube
Pediatric- 2ml; red top tube
Testing
Schedule:
Bi-weekly (Tues and Fri) - Batch analysis performed 
twice weekly on Tuesdays and Fridays excluding 
university holidays.
Analytic Time:
4 days
Reference Range:
A normal serum sample shows a light diffused staining of polyclonal 
immunoglobulins in all tracks. The serum IFE report will include a 
descriptive report of abnormality, if present.
Comments:
Include relevant clinical information and consultation request.  
Quantitative kappa/lambda light chain ratios are recommended
as a screen for suspected multiple myeloma, Waldenstrom's
macroglobulinemia, or malignant lymphomas, except in the setting of
bone marrow or solid organ transplantation.
Methodology:
Immunofixation Electrophoresis
Sample
Processing:
Centrifuge at 3500 RPM for 5 minutes.
Aliquot serum into labeled container and cap.
Relevant clinical information must be submitted with specimen in order 
to provide correct interpretation of test results.
Sample
Storage:
Refrigerate serum 2-8 degree C.
Transport
Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at ambient temperature.
CPT Code:
86334 x1 (Technical)
86334-26 x1 (Professional)

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Updated: 01/09/2008