Amikacin
| Order Code: | AMIK |
| Order Form: | Laboratory Requisition |
Specimen:
Plasma
Collection Medium:
 |
| Plasma Separator Tube |
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood in light green top or ONE microtainer for pediatric
patients.
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
1 hour (upon receipt in laboratory)
Reference Range:
Peak 20-25 mcg/mL, trough 5-10 mcg/mL. Peak levels: 45-75 Min after
I.M.
Dose; 15-30 min. After I.V. Dose. Trough levels: Not more than 30 min
before next dose.
Critical value: >35 mcg/mL
Comments:
Kanamycin interferes with measurement of amikacin, giving falsely
elevated results.
Test
Limitations:
Patient samples which contain kanamycin A, kanamycin B, 3', or 4'
dideoxykanamycin B will yield falsely elevated values for amikacin.
However, these drugs are not usually coadministered with amikacin.
High concentration of penicillins or cephalosporins have been shown to
inactivate aminoglycosides in vitro. The degree of inactivation is
dependent on the particular aminoglycoside being measured, the type and
concentration of the penicillin or cephalosporin that is also present
and the storage conditions of the sample. Samples from patients
receiving additional antibiotics of these types should be assayed
immediately or stored frozen.
Hemolysis: No significant interference up to an H index of 1000 (approximate hemoglobin concentration: 1000 mg/dL).
Lipemia: No significant interference up to an L index of 2000 (approximate triglycerides concentration: 4000 mg/dL). Note there is poor correlation between turbidity and triglyceride concentration.
Icterus: No significant interference up to an I index of 50 (approximate conjugated and unconjugated bilirubin concentration: 50 mg/dL).
Hemolysis: No significant interference up to an H index of 1000 (approximate hemoglobin concentration: 1000 mg/dL).
Lipemia: No significant interference up to an L index of 2000 (approximate triglycerides concentration: 4000 mg/dL). Note there is poor correlation between turbidity and triglyceride concentration.
Icterus: No significant interference up to an I index of 50 (approximate conjugated and unconjugated bilirubin concentration: 50 mg/dL).
Methodology:
Kinetic Interaction of Microparticles in Solution (KIMS)
Sample
Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Specimen must be frozen immediately.
Label transport tube with two patient identifiers, date and time of collection.
Aliquot plasma into labeled container and cap.
Specimen must be frozen immediately.
Label transport tube with two patient identifiers, date and time of collection.
Sample
Storage:
Freeze specimen immediately.
Transport
Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Place dry ice on top of specimen in styrofoam container.
Place requisition into outside pocket of bag.
Place dry ice on top of specimen in styrofoam container.
CPT Code:
80150
See Additional Information:
Chemistry Critical Lab Values
Chemistry Critical Lab Values