Immunoglobulin E (IgE), Individual Quant

Label Mnemonic:	IGE
Epic code:	LAB74
Order form:	Laboratory Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	82785

Specimen(s):

Plasma

Collection Medium:

Plasma Separator Tube 4.5 mL

Alternate Collection Media:

Call laboratory for additional acceptable specimen collection containers.

Minimum:

3 mL whole blood from light green top tube or TWO Microtainer^® devices

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Turn Around Time:

1 hour (upon receipt in laboratory)

Reference Range:

Non-allergic Adult:    0-100 IU/mL
Non-allergic Children: 10-20% of adult values

Neonates:  0.0-1.5 IU/mL
Infants in 1st year of life:  0-15 IU/mL
Children aged 1-5 years:  0-60 IU/mL
Children aged 6-9 years:  0-90 IU/mL
Children aged 10-15 years:  0-200 IU/mL

Comments:

This assay may be significantly impacted by high-dose biotin (>5 mg dose) taken within previous 12 hours. High concentrations of biotin may lead to falsely decreased results. These concentrations may be found in patients taking over-the-counter supplements with biotin content much higher than nutritional requirements for biotin. Specimens should not be collected until at least 12 hours after the last dose.

Test Limitations:

The assay is unaffected by icterus (bilirubin is less than 37 
mg/dL), hemolysis (Hb is less than 100 mg/dL), lipemia (triglycerides 
is less than 2200 mg/dL) and biotin is less than 100 ng/mL. 
(criterion: recovery within plus or minus 10% of initial value).

No interference was observed from rheumatoid factor up to 6000 U/mL 
(method comparison: Elecsys IgE and a comparison IgE test on 50 
samples).

No high dose hook effect at IgE concentrations of up to 50 000 IU/mL 
(120000 ng/mL).

In vitro tests were performed on 36 commonly used pharmaceuticals. No 
interference with the assay was found.

As with all tests containing monoclonal mouse antibodies, erroneous 
findings may be obtained from samples taken from patients who have 
been treated with monoclonal mouse antibodies or have received them 
for diagnostic purposes.

In rare cases interference due to extremely high titers of antibodies 
to ruthenium can occur.

IgE contains additives which minimize these effects.

Extremely high titers of antibodies to streptavidin can occur in 
isolated cases and cause interference.

For diagnostic purposes, the IgE findings should always be assessed in 
conjunction with the patient's medical history, clinical examination 
and other findings.

Methodology:

Electrochemiluminescence Immunoassay

Sample Processing:

Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.

Sample Storage:

Refrigerate.

Transport Instructions:

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

82785

See Additional Information:
Biotin Interference with Immunoassays