Alpha-1-Antitrypsin Quantitation
| Order Code: | A1AT |
| Epic Lab Code: | LAB927 |
| Order Form: | A-1a General Lab or Epic Req |
Chemistry
6240 RCP
356-3527
6240 RCP
356-3527
Specimen:
Plasma
Collection Medium:
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| Plasma Separator Tube |
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood in light green top tube or ONE microtainer
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around
Time:
1 hour (upon receipt in laboratory)
Reference Range:
100-200 mg/dL
A1AT is an acute phase reactant, therefore any inflammatory process may
result in transient elevations of the patient's A-1-AT level.
Test
Limitations:
Criterion: Recovery within plus or minus 10% of initial values.
Icterus: No significant interference up to an I index of 60
(approximate conjugated and unconjugated bilirubin concentration: 60
mg/dL).
Hemolysis: No significant interference up to an H index of 1000
(approximate hemoglobin concentration: 1000 mg/dL).
Lipemia (Intralipid): No significant interference up to an L index of
890 (approximate triglycerides concentration: 1780 mg/dL). There is
poor correlation between turbidity and triglycerides concentration.
Rheumatoid factors less than 100 IU/mL do not interfere.
No high dose hook effect is seen up to an alpha 1-antitrypsin
concentration of 1200 mg/dL.
Elevated estrogen levels (oral contraceptives; third trimester of
pregnancy) give rise to false positive results. CRP and haptoglobin
should therefore also be determined.*
*Laurell CB, Kullander S, Thorell J. Effect of administration of a
combined estrogen-progestin contraceptive on the level of individual
plasma proteins. Scand J Lab Invest 1968;21:337-343.
Methodology:
Immunoturbidometric
CPT Code:
82103
