The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK

Fragile X, DNA Testing
Order Code: FX
Order Form: A-1a Molecular Pathology/Diagnostics or IPR Req 		  Molecular Pathology
6004 BT GH
384-9568 
Specimen
 
Whole Blood
Collection Medium:
Lavender top tube (EDTA) 


Minimum:


Adult minimum: 3 mL whole blood in lavender top tube (EDTA)
Children minimum: 2 mL whole blood in lavender top tube (EDTA)
Testing on smaller volumes than those requested will be attempted.  
However, in some cases, small blood volumes may compromise the ability 
to perform testing.
Testing requires a dedicated collection tube.


Delivery Instructions:


Room temperature up to 24 hours.  Refrigerate overnight, 
weekends and holidays.


Testing Schedule:


Weekly


Analytic Time:


21 days


Reference Range:


Normal: < or = to 44 CGG repeats
Indeterminate:  45-55 CGG repeats
Pre mutation (carrier):  56-199 CGG repeats
Full mutation (affected):  > or = to 200 CGG repeats


Methodology:


Polymerase Chain Reaction (PCR) and Southern Blot


CPT Code:


83890, 83892 (x2), 83894 (x2), 83896, 83897, 83898, 83912




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Updated: 02/19/2007

Note: The information contained in this handbook is  
            for use by personnel of University of Iowa Health Care. 
            No other use is implied or intended.