Estradiol (E2)
| Label Mnemonic: | EDIOL |
| Epic code: | LAB523 |
| Downtime form: | A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery |
Chemistry
6240 RCP
356-3527
6240 RCP
356-3527
Specimen(s):
Plasma
Collection Medium:
 |
| Plasma Separator Tube 4.5 mL |
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood from light green top tube or TWO
Microtainer®
devices
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around
Time:
1 hour (upon receipt in laboratory)
Reference Range:
Males (18+ years) 11 - 43 pg/mL Females, non-pregnant Follicular Phase 12 - 233 pg/mL Ovulation Phase 41 - 398 pg/mL Luteal Phase 22 - 341 pg/mL Postmenopause <5 - 138 pg/mL Pregnancy 1st Trimester 154 - 3,243 pg/mL 2nd Trimester 1,561 - 21,280 pg/mL 3rd Trimester 8,525 - >30,000 pg/mL Children (1-10 years) Boys <5 - 20 pg/mL Girls 6 - 27 pg/mL
Comments:
This assay may be significantly impacted by high-dose biotin (>5 mg
dose) taken within previous 12 hours. High concentrations of biotin
may lead to falsely increased results. These concentrations may be
found in patients taking over-the-counter supplements with biotin
content much higher than nutritional requirements for biotin.
Specimens should not be collected until at least 12 hours after the
last dose.
Assay methodology changed to Roche Diagnostics Estradiol III assay on April 20, 2015. Adult reference ranges updated as part of change in methodology. Adult male reference range further updated on March 7, 2017.
This test is also performed in the Iowa River Landing (IRL) clinical laboratory (for specimens drawn at that site).
Assay methodology changed to Roche Diagnostics Estradiol III assay on April 20, 2015. Adult reference ranges updated as part of change in methodology. Adult male reference range further updated on March 7, 2017.
This test is also performed in the Iowa River Landing (IRL) clinical laboratory (for specimens drawn at that site).
Test
Limitations:
The assay is unaffected by icterus (bilirubin is less than 66 mg/dL),
hemolysis (Hb is less than 1000 mg/dL), lipemia (Intralipid is less
than 1000 mg/dL) and biotin is less than 36 ng/mL. (criterion:
recovery within plus or minus 10% of initial value). No interference
was observed from rheumatoid factors up to a concentration of 1200
U/mL.
The estradiol assay shows significant cross-reactivity with fulvestrant, a medication used to treat breast cancer. Contact commercial mailouts (319-356-8593) if estradiol measurement is needed in a patient currently receiving fulvestrant. In rare cases interference due to extremely high titers of antibodies to streptavidin and ruthenium can occur. Estradiol III contains additives which minimize these effects. For diagnostic purposes, the Estradiol III findings should always be assessed in conjunction with the patient's medical history, clinical examination and other findings.
The estradiol assay shows significant cross-reactivity with fulvestrant, a medication used to treat breast cancer. Contact commercial mailouts (319-356-8593) if estradiol measurement is needed in a patient currently receiving fulvestrant. In rare cases interference due to extremely high titers of antibodies to streptavidin and ruthenium can occur. Estradiol III contains additives which minimize these effects. For diagnostic purposes, the Estradiol III findings should always be assessed in conjunction with the patient's medical history, clinical examination and other findings.
Methodology:
Electrochemiluminescence Immunoassay
CPT Code:
82670
See Additional Information:
Biotin Interference with Immunoassays
Biotin Interference with Immunoassays