Specimen

Plasma

Collection Medium:

Alternate
Collection Media:

Call laboratory for additional acceptable specimen collection 
containers.

Minimum:

2 ml whole blood from light green top tube two 0.6 microtubes

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Analytic Time:

2 hours (upon receipt in laboratory)

Reference Range:

MALES                 7.63 - 42.6 pg/ml

FEMALES:
  Follicular Phase    12.5 - 166 pg/ml
  Ovulation Phase     85.9 - 498 pg/ml
  Luteal Phase        43.8 - 211 pg/ml

  Postmenopause       <5.0 - 54.7 pg/ml

  Pregnancy
    1st trimester       38 - 3175 pg/ml
    2nd trimester      678 - 16633 pg/ml
    3rd trimester       43 - 33781 pg/ml

CHILDREN (1-10 YEARS)
    Boys              <5.0 - 20.0 pg/ml
    Girls              6.0 - 27.0 pg/ml

Comments:

New analytical immunoassay with different reference ranges instituted 
3/13/00 at 07:00.

Test
Limitations:

The assay is unaffected by icterus (bilirubin is less than 66 mg/dl), 
hemolysis (Hb is less than 1.0 g/dl), lipemia  (Intralipid is less than 
1000 mg/dl) and biotin is less than 36 ng/ml. (criterion: recovery 
within plus or minus 10% of initial value).  In patients receiving 
therapy with high biotin doses (i.e. is greater than 5 mg/day) no 
sample should be taken until at least 8 hours after the last biotin 
administration.

No interference was observed from rheumatoid factors up to a 
concentration of 1200 U/ml.  In vitro tests were performed on 18 
commonly used pharmaceuticals. No interference with the
assay was found.

The risk of interference from potential immunological interactions 
between test components and rare sera has been minimized by the 
inclusion of suitable additives.

In rare cases interference due to extremely high titers of antibodies 
to streptavidin and ruthenium can occur.  Estradiol II contains 
additives which minimize these effects.

For diagnostic purposes, the Estradiol II findings should always be 
assessed in conjunction with the patient's medical history, clinical 
examination and other findings.

Methodology:

Electrochemiluminescence Immunoassay

CPT Code:

82670