Collect TWO full red top or lavender top tubes. Recommended minimum: 4.0 mL serum or plasma Absolute minimum: 2.0 mL serum or plasma

Unexposed non-tobacco user Passive exposure Nicotine Less than 2 ng/mL Less than 2 ng/mL Cotinine Less than 2 ng/mL Less than 8 ng/mL 3-OH-Cotinine Less than 2 ng/mL Less than 2 ng/mL Abstinent user for more Active tobacco product use than 2 weeks Nicotine Less than 2 ng/mL 30 - 50 ng/mL Cotinine Less than 2 ng/mL 200 - 800 ng/mL 3-OH-Cotinine Less than 2 ng/mL 100 - 500 ng/mL Reference range source: Clinical Chemistry 48:9 1460-1471, 2002

1. Drugs covered: nicotine, cotinine, 3-OH-cotinine. 2. Positive cutoff: 2 ng/mL The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. For medical purposes only; not valid for forensic use.