Cortisol/Cortisone Urine Free (by LC-MS/MS)
| Order Code: | UFCHPLC |
| Epic Lab Code: | LAB382 |
| Order Form: | A-1a General Lab or Epic Req |
Commercial Mail-out Laboratory
6240 RCP
356-3527
6240 RCP
356-3527
Specimen:
Urine More information
Collection Medium:
) |
| Urine - 24 hour/timed plastic urine container |
Minimum:
Preferred Minimum: 4 mL aliquot from well mixed 24 hr sample.
Absolute Minimum: 1 mL aliquot from well mixed 24 hr sample.
Absolute Minimum: 1 mL aliquot from well mixed 24 hr sample.
Rejection Criteria:
Room temperature specimens. Acidified specimens or specimens with
preservatives.
Delivery Instructions:
Submit specimen to laboratory as soon as possible after
collection period is completed.Specimen
Instructions:
Record start and end of 24 hour collection on requisition. Urine must
be refrigerated during collection.
Analytic Time:
2 working days upon receipt at reference laboratory
Reference Range:
Cortisol (μg/day)
Female
3-8 years: Less than 18 μg/day
9-12 years: Less than 37 μg/day
13-17 years: Less than 56 μg/day
18 years and older: Less than 45 μg/day
Male
3-8 years: Less than 18 μg/day
9-12 years: Less than 37 μg/day
13-17 years: Less than 56 μg/day
18 years and older: Less than 60 μg/day
Cortisol/Cortisone, Ratio*
Female
0-17 years: To be determined
18 years and older: 0.15-0.50
Male
0-17 years: To be determined
18 years and older: 0.15-0.50
Cortisone
Female
Value used to calculate Cortisol/Cortisone ratio
Cortisol (μg/g crt)
Female
Prepubertal: Less than 25 μg/g crt
18 years and older: Less than 24 μg/g crt
Pregnancy: Less than 59 μg/g crt
Male
Prepubertal: Less than 25 μg/g crt
18 years and older: Less than 32 μg/g crt
The urine cortisol-to-creatinine ratio will be reported when the urine
collection is random, other than 24 hours, or the urine volume is less
than 400 mL/24 hours.
Low urinary cortisol concentrations may be consistent with adrenal
insufficiency.
Comments:
Reference intervals for children are based on literature from Taylor
R.L. et al., Validation of a High-Throughput Liquid Chromatography-
Tandem Mass Spectrometry Method for Urine Cortisol and Cortisone.
Clinical Chemistry 2002; 48:1511-19.
*The ratio of the concentrations of cortisol to cortisone will not be evaluated if the cortisol concentration is less than 5 μg/L.
*The ratio of the concentrations of cortisol to cortisone will not be evaluated if the cortisol concentration is less than 5 μg/L.
Methodology:
Quantitative High Performance Liquid Chromatography-Tandem Mass
Spectrometry
CPT Code:
Cortisol 82530
LC-MS/MS 83789