KIT (GIST) Targeted Gene Analysis Exons 9, 11, 13, 17 with Interpretation
Order Code: KIT
Epic Lab Code: LAB7660
Order Form: A-1a Molecular Pathology/Diagnostics or Epic Req
Molecular Pathology
6004 BT GH
384-9568
Specimen(s):
Tissue
Formalin fixed, paraffin embedded tissue block or 1 H&E with 10 unstained slides 5-10 micron-thick on uncharged slides at 20-25°C.
Minimum:
Specimen requires 20% or greater tumor cell content. Submit 1 H&E with 10 unstained 6 micron-thick sections on non-charged slides or formalin- fixed, paraffin-embedded tissue block.
Rejection Criteria:
Specimens fixed in B5 fixative or that have been decalcified will not be accepted. Tumor specimens containing less than 20% tumor cells or are less than 10mm2 in area may be unacceptable.
Delivery Instructions:
Testing is generally not available on a STAT basis. However, expedited testing can be arranged by contacting the Molecular Pathology Laboratory at 384-9568 or the Molecular Genetic Pathology Fellow (pager #8682).
Testing Schedule:
0800-1700 Testing offered once per week excluding weekends and University holidays.
Turn Around Time:
7-10 working days
Reference Range:
Negative
Comments:
The principle use of this test is to detect mutations in patients with gastrointestinal stromal tumor (GIST) and melanoma. For acute myeloid leukemia (AML) testing, please order KITAML Mutation Analysis (LAB7659). This test is not intended to detect the D816 mutation in bone marrow for mast cell disease or to detect minimal residual disease, see KITMAST Mast Cell Disease (LAB7567).
Methodology:
PCR followed by DNA sequencing.
CPT Code:
81404
 
See also:
KIT (AML) Targeted Gene Analysis Exons 8, 17 with Interpretation, Whole Blood, Bone Marrow
KIT Mutation (D816V) For Mast Cell Disease, Whole Blood, Bone Marrow or Tissue