The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Disaccharidase Analysis
Order Code: DISAC
Epic Lab Code: LAB5822
Order Form: A-1a Miscellaneous Request or Epic Req
  Commercial "Mail-out" Laboratory
6240 RCP
356-3527
Specimen:
Small Bowel
Collection Medium:
Miscellaneous container; contact laboratory
Minimum:
5 mg of small bowel
Specimen
Instructions:
Approximately 5 mg of small bowel should be collected through endoscopy 
for the complete enzyme analysis.

The tissue should be placed on the wall of a small, tightly capped, 
plastic tube appropriately labeled with the patient name, date of 
birth, ID number, date specimen collected and referring physician name.  
A 1.5 mL microtube work well.  PLEASE DO NOT PLACE THE TISSUE ON GAUZE, 
FILTER PAPER, OR USE ANY TYPE OF SUPPORT MEDIUM.
Analytic Time:
3 days
Comments:
Please print, complete and submit the Request Form from JOLI 
Diagnostics to the lab, with the specimen and the A-1a Miscellaneous 
Request.
Methodology:
Mucosal tissue is homogenized and incubated with the appropriate 
disaccharide substrate.  Enzyme hydrolysis results in the formation of 
glucose.  A quantitative measurement is then done on the glucose 
yielded.
CPT Code:
82945 (x4)

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Updated: 03/18/2009

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.